Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins
Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins
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DOI：
10.1016/j.jchromb.2008.04.016
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发表时间：
2008-05-15
影响因子：
3
通讯作者：
Liu, Chun-Zhao
中科院分区：
文献类型：
--
作者：
Zhang, Bin;Yang, Ruiyuan;Liu, Chun-Zhao
绿原酸是中草药金银花中富含的最具生物活性的化合物之一，是一种天然抗氧化剂，具有抗炎、抗肿瘤、抗突变和抗癌等作用。建立了金银花粗提物中绿原酸的高效分离工艺。HPD-850树脂对绿原酸的吸附容量和吸附解吸率最高，25 ℃时的吸附符合Langmuir等温式。HPD-850树脂的吸附容量强烈依赖于初始吸附液的pH值。采用HPD-850树脂填充柱对金银花粗提物中绿原酸进行动态吸附和解吸实验，优化了金银花粗提物中绿原酸的分离工艺。经HPD-850树脂处理一次，绿原酸含量由11.2%提高到50.0%，提高了4.46倍，回收率为87.9%。采用HPD-850大孔吸附树脂对绿原酸进行吸附和解吸，可实现绿原酸的高效制备分离，为绿原酸的大规模分离纯化提供了一条新的途径。(c)2008 Elsevier B. V.保留所有权利。
Chlorogenic acid, one of the most bioactive compounds rich in the Chinese medicinal herb honeysuckle, is a natural antioxidant and serves as anti-inflammatory, anti-tumor, anti-mutagenic and anti-carcinogenic agent. An efficient preparative separation process of chlorogenic acid from honeysuckle crude extracts has been developed in the present study. HPD-850 resin offers the best adsorption capacity, and adsorption and desorption ratios for chlorogenic acid among the nine macroporous resins tested, and its adsorption rate at 25 degrees C fit best to the Langmuir isotherm. The adsorption capacity of HPD-850 resin was found to depend strongly on the pH value of the initial adsorption solution. The dynamic adsorption and desorption experiments have been carried out on a HPD-850 resin packed column to optimize the separation process of chlorogenic acid from honeysuckle crude extracts. After one run treatment with HPD-850 resin, the chlorogenic acid content in the final product was increased 4.46-fold from 11.2% to 50.0%, with a recovery yield of 87.9%. The preparative separation of chlorogenic acid can be easily and efficiently achieved via adsorption and desorption on HPD-850 resin, and the method developed will provide a potential approach for large-scale separation and purification of chlorogenic acid for its wide pharmaceutical use. (c) 2008 Elsevier B.V. All rights reserved.