Infections of nervous necrosis virus in wild and cage-reared marine fish from South China Sea with unexpected wide host ranges
Infections of nervous necrosis virus in wild and cage-reared marine fish from South China Sea with unexpected wide host ranges
复制标题
南海野生和网箱养殖海水鱼类神经坏死病毒感染，宿主范围意外广泛
DOI：
10.1111/jfd.12265
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发表时间：
2015-06-01
影响因子：
2.5
通讯作者：
Lin, L.
中科院分区：
文献类型：
--
作者：
Liu, X. D.;Huang, J. N.;Lin, L.
神经坏死病毒（NNV）感染对野生鱼类的影响引起了人们的关注。本文介绍了对中国南海野生和网箱养殖海水鱼类的NNV进行季度调查的结果。采集野生鱼类69种892份和网箱养殖鱼类11种381份样品，采用巢式PCR和双套式PCR方法进行检测。在巢式PCR的情况下，在野生鱼和笼养鱼样品中分别检测到3.0%和3.1%的阳性信号。巢式RT-PCR检测野生鱼和笼养鱼的阳性率分别为42.3%和63.0%。若以鱼类为研究对象，野生鱼和笼养鱼的PCR检测阳性率分别为21.7%和72.7%。巢式RT-PCR检测野生鱼和笼养鱼的阳性率分别为65.2%和100%。测定巢式PCR产物的核苷酸序列。系统进化树分析表明，所有分离株均属于赤点石斑鱼神经坏死病毒（RGNNV）基因型。35种海洋鱼类是NNV的新宿主。
The concerns about the impact of the nervous necrosis virus (NNV) infections in wild fish have been raised. This paper presents the results of quarterly surveys of NNV in wild and cage-reared marine fish from South China Sea. Samples of 892 wild fish belonging to 69 species and 381 cage-reared fish belonging to 11 species were collected and were detected by seminested PCR and nested PCR. In the case of seminested PCR, the positive signal was detected in 3.0% and 3.1% samples of wild and cage-reared fish, respectively. However, by nested RT-PCR, the positive signal was observed in 42.3% and 63.0% samples of wild and cage-reared fish, respectively. If the fish species were considered, the positive signal was detected in 21.7% and 72.7% species of wild and cage-reared fish by seminested PCR assay, respectively. However, by nested RT-PCR, the positive signal was observed in 65.2% and 100% species of wild and cage-reared fish, respectively. The nucleotide sequences of the nested PCR products were determined. Phylogenetic tree showed that all the obtained viral isolates belonged to the red-spotted grouper nervous necrosis virus (RGNNV) genotype. Thirty-five species of the marine fish were the new hosts of NNV.