Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis
Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis
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DOI：
10.1016/j.plantsci.2005.03.030
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发表时间：
2005-08-01
期刊：
影响因子：
5.2
通讯作者：
Ezaki, B
中科院分区：
文献类型：
--
作者：
Katsuhara, M;Otsuka, T;Ezaki, B
在盐胁迫下对拟南芥根中活性氧（ROS）相关的膜脂过氧化进行荧光可视化和研究。在没有盐胁迫的对照根中，在伸长区域比在分生组织区域观察到更多的荧光。 100 mM NaCl 的盐胁迫增强了两者的荧光，表明盐胁迫诱导了 ROS，从而导致膜脂过氧化。在过表达拟南芥（parB植物）的转基因烟草谷胱甘肽S-转移酶中，观察到比非转基因对照植物更少的荧光。在盐胁迫的parB植物根部中，分生组织区域的荧光亮度降低至非转基因植物的46%。然而，在pH 5.7的盐胁迫下，parB植物对根生长的抑制并未得到改善。也就是说，100 mM的盐胁迫使亲本对照植物和parB植物的根生长降低至40%或更低。两个测试品系之间的根组织渗透压几乎相同，这可能是两个品系之间的根系生长没有观察到差异的原因之一。这些结果表明，盐胁迫诱导的氧化应激以及谷胱甘肽S-转移酶基因的引入/过度表达可能减少了ROS的量，但ROS的去除不足以影响耐盐性，因为盐胁迫还会导致渗透不平衡，从而减少根系生长。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Reactive oxygen species (ROS)-related membrane lipid peroxidation in the root of Arabidopsis thaliana was fluorescently visualized and investigated under salt stress. In the control roots without salt stress, more fluorescence was observed in the elongating region than in the meristematic region. Salt stress of 100 mM NaCl enhanced the fluorescence in both, indicating that salt stress-induced ROS, and consequently membrane lipid peroxidation. In transgenic tobacco glutathione S-transferase over-expressing Arabidopsis (the parB plants), less fluorescence was observed than in the non-transgenic control plants. In the salt-stressed parB plant roots, the fluorescent brightness was reduced to 46% of that of the non-transgenic plant in the meristematic region. However, the inhibition of root growth was not improved in parB plants under salt stress at pH 5.7. That is, 100 mM of salt stress reduced the root growth to 40% or less both in the parent control plants and the parB plants. The root tissue osmotic pressure was almost the same between the two tested lines, which may be one of the reasons why no difference was observed in root growth between the two lines. These results suggested that salt stress-induced oxidative stress, and the introduction/overexpression of a glutathione S-transferase gene may have reduced the amount of ROS but the removal of ROS was not sufficient to effect salt tolerance because salt stress also caused an osmotic imbalance reducing the root growth. (c) 2005 Elsevier Ireland Ltd. All rights reserved.