Transcriptional Control in Marine Copiotrophic and Oligotrophic Bacteria with Streamlined Genomes
Transcriptional Control in Marine Copiotrophic and Oligotrophic Bacteria with Streamlined Genomes
复制标题
DOI：
10.1128/aem.01299-16
复制
发表时间：
2016-10-01
影响因子：
4.4
通讯作者：
Kirchman, David L.
中科院分区：
文献类型：
--
作者：
Cottrell, Matthew T.;Kirchman, David L.
细菌通常通过转录控制对环境刺激做出反应，但对于海洋细菌来说可能并非如此，例如“Candidatus Pelagibacter ubique”，它是海洋中最丰富的生物SAR 11进化枝的培养代表。这种细菌具有小的流线型基因组和异常低数量的转录调节因子，这表明在Pelagibacter中转录控制较低，并限制了其对环境条件的反应。分批培养生长期间的转录组测序显示，在Pelagibacter和另一种寡养菌（SAR92）中，只有0.1%的蛋白质编码基因似乎处于转录控制之下，而在兼养菌Polaribacter sp.菌株MED 152和Ruegeria pomeroyi中，>10%的基因处于转录控制之下。当生长水平改变时，Pelagibacter和SAR 92的转录水平保持稳定，但在MED 152和R.波默罗伊。使用内部RNA测序标准品测定的每个细胞的转录本丰度较低（
Bacteria often respond to environmental stimuli using transcriptional control, but this may not be the case for marine bacteria such as "Candidatus Pelagibacter ubique," a cultivated representative of the SAR11 clade, the most abundant organism in the ocean. This bacterium has a small, streamlined genome and an unusually low number of transcriptional regulators, suggesting that transcriptional control is low in Pelagibacter and limits its response to environmental conditions. Transcriptome sequencing during batch culture growth revealed that only 0.1% of protein-encoding genes appear to be under transcriptional control in Pelagibacter and in another oligotroph (SAR92) whereas >10% of genes were under transcriptional control in the copiotrophs Polaribacter sp. strain MED152 and Ruegeria pomeroyi. When growth levels changed, transcript levels remained steady in Pelagibacter and SAR92 but shifted in MED152 and R. pomeroyi. Transcript abundances per cell, determined using an internal RNA sequencing standard, were low (