Hepatic genomic assessment of dietary ingestion of 2-aminoanthracene in Sprague Dawley rats
Hepatic genomic assessment of dietary ingestion of 2-aminoanthracene in Sprague Dawley rats
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DOI：
10.1080/03601234.2023.2185023
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发表时间：
2023-03-06
影响因子：
2
通讯作者：
Gato，Worlanyo E. E.
中科院分区：
文献类型：
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作者：
Cisse，Awa M. M.;Erber，Jody E. E.;Gato，Worlanyo E. E.
本研究旨在探讨多环芳烃（PAH）2-氨基蒽（2-AA）对肝脏的影响。PAH是化石燃料不完全燃烧的副产品。具体而言，已报告了2-AA对动物不同身体组织的影响。肝脏是多环芳烃（包括2-AA）代谢的中心器官。Sprague道利大鼠在其饮食中摄入明确剂量的2-AA（0、50和100 mg/kg 2-AA），持续12周。使用AffyestivalRatGenome2302.0微阵列进行肝脏整体基因表达。总共有超过17，000个基因表达。当对照组大鼠与低剂量动物相比时，大约70个基因上调，而65个基因下调。同样，高浓度2-AA组与对照组大鼠相比，103个基因表达上调，49个基因表达下调。该结果表明，基因表达倍数变化的幅度取决于摄入的2-AA的剂量。一些差异表达的基因参与生物过程，如基因转录，细胞周期和免疫系统功能，表明摄入2-AA可能会影响这些过程。观察到肝脏炎症、非酒精性肝病、肝脏葡萄糖加工和PAH代谢相关基因的过度表达。
This research aims to investigate the effects of 2-aminoanthracene (2-AA), a polycyclic aromatic hydrocarbon (PAH), on the liver. PAH is a by-product of the incomplete combustion of fossil fuels. Specifically, the impact of 2-AA on different body tissues in animals has been reported. The liver is an organ central to the metabolism of PAHs, including 2-AA. Sprague Dawley rats ingested a well-defined dose of 2-AA in their diet (0, 50, and 100 mg/kg 2-AA) for 12 weeks. Hepatic global gene expression using Affymetrix Rat Genome 230 2.0 microarray was performed. Overall, more than 17,000 genes were expressed. Approximately 70 genes were upregulated, while 65 were downregulated when control rats were compared with low-dose animals. Similarly, 103 genes were upregulated and 49 downregulated when the high-concentration 2-AA group was compared with the control group rats. This result suggests that the magnitude of gene expression fold change depends on the dose of 2-AA ingested. Several differentially expressed genes are involved in biological processes such as gene transcription, cell cycle, and immune system function, indicating that the ingestion of 2-AA could impact these processes. The over-expression of genes related to liver inflammation, nonalcoholic liver disease, hepatic glucose processing, and PAH metabolism were noted.