Molecular and geographic analyses of vampire bat-transmitted cattle rabies in central Brazil
Molecular and geographic analyses of vampire bat-transmitted cattle rabies in central Brazil
复制标题
DOI：
10.1186/1746-6148-4-44
复制
发表时间：
2008-11-05
影响因子：
2.6
通讯作者：
Sakai, Takeo
中科院分区：
文献类型：
--
作者：
Kobayashi, Yuki;Sato, Go;Sakai, Takeo
背景资料：吸血蝙蝠是重要的狂犬病病毒载体，在拉丁美洲的畜牧业和公共卫生部门造成严重问题。为了评估吸血蝙蝠传播的狂犬病的流行病学特征，作者进行了系统发育和地理分析，使用大量的牛狂犬病病毒分离株的序列数据收集了广泛的地理区域在Brazils.Methods：部分核蛋白基因的狂犬病病毒分离自666头牛和18吸血蝙蝠1987年至2006年进行测序和系统发育分析。的遗传变异绘制在地形图的Brazils.Results：在这项研究中，593个样本组成的24个遗传变异进行了分析。观察到变异体的区域定位，发现几种变异体的分布由山脉作为地理边界划定。被归类为相同的系统发育组的吸血蝙蝠和牛的分离株的地理分布被发现重叠，具有很高的确定性。在这项研究中分析的大多数样品被隔离从相邻地区连接rives.Conclusion：这项研究揭示了存在几十个区域变异与吸血蝙蝠在巴西，这些变种的分布模式被发现受山脉和河流。这些结果表明，吸血蝙蝠相关的狂犬病的流行病学特征似乎与地形和地理特征的地区，保持牛，影响吸血蝙蝠生态的因素。
Background: Vampire bats are important rabies virus vectors, causing critical problems in both the livestock industry and public health sector in Latin America. In order to assess the epidemiological characteristics of vampire bat-transmitted rabies, the authors conducted phylogenetic and geographical analyses using sequence data of a large number of cattle rabies isolates collected from a wide geographical area in Brazil.Methods: Partial nucleoprotein genes of rabies viruses isolated from 666 cattle and 18 vampire bats between 1987 and 2006 were sequenced and used for phylogenetic analysis. The genetic variants were plotted on topographical maps of Brazil.Results: In this study, 593 samples consisting of 24 genetic variants were analyzed. Regional localization of variants was observed, with the distribution of several variants found to be delimited by mountain ranges which served as geographic boundaries. The geographical distributions of vampire-bat and cattle isolates that were classified as the identical phylogenetic group were found to overlap with high certainty. Most of the samples analyzed in this study were isolated from adjacent areas linked by rivers.Conclusion: This study revealed the existence of several dozen regional variants associated with vampire bats in Brazil, with the distribution patterns of these variants found to be affected by mountain ranges and rivers. These results suggest that epidemiological characteristics of vampire bat-related rabies appear to be associated with the topographical and geographical characteristics of areas where cattle are maintained, and the factors affecting vampire bat ecology.