A label-free G-quadruplex DNA-based fluorescence method for highly sensitive, direct detection of cisplatin
A label-free G-quadruplex DNA-based fluorescence method for highly sensitive, direct detection of cisplatin
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DOI：
10.1016/j.snb.2014.05.027
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发表时间：
2014-10
影响因子：
8.4
通讯作者：
Hualin Yang;Cui Hanli;Longgang Wang;Li Yan;Yong Qian;X. Zheng;Wei Wei-Wei;J. Zhao
中科院分区：
文献类型：
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作者：
Hualin Yang;Cui Hanli;Longgang Wang;Li Yan;Yong Qian;X. Zheng;Wei Wei-Wei;J. Zhao
顺铂是一种广泛应用的抗癌药物，其快速、简便的检测方法备受关注。基于顺铂与G-四链体DNA的相互作用，我们建立了一种简单的无标记荧光快速检测顺铂的方法。滴定实验表明，顺铂浓度与荧光信号变化率（F0/F）在1 ~ 10 μM范围内呈线性关系，检测限为720 nM，甚至低于化疗患者尿液中顺铂的常见浓度（54.3 ~ 321 μM）。顺铂结合的平衡解离常数KD值确定为1.19 × 10− 5 M。这一结果表明，我们的方法对顺铂具有相对较高的亲和力，可以结合溶液中的微摩尔浓度的顺铂。我们的方法也显示出明显的选择性之间的测试药物，甚至顺铂和奥沙利铂/卡铂。我们证明了这种方法的高灵敏度，在直接检测尿液样本中的顺铂和顺铂在活细胞中的荧光成像。
Cisplatin is a widely used anticancer chemotherapeutic, and fast, convenient detection methods for it are highly desirable. Based on the interaction of cisplatin with G-quadruplex DNA, we developed a simple label-free fluorescence method for rapid cisplatin detection. The titration experiment showed that the cisplatin concentration and the fluorescence signal change ratios (F0/F) exhibited a consistent linear correlation within the 1 to 10 μM range with a limit of detection of 720 nM, which was even lower than the common concentration of cisplatin in chemotherapy patients’ urine (54.3 to 321 μM). The equilibrium dissociation constantKDvalue for cisplatin binding was determined to be 1.19 × 10−5M. This result demonstrated that our method had relatively high affinity toward cisplatin and could bind micromolar concentrations of cisplatin in solution. Our method also shows obvious selectivity among tested drugs, even between cisplatin and oxaliplatin/carboplatin. We demonstrated the high sensitivity of this methodology in the direct detection of cisplatin in urine samples and the fluorescence imaging of cisplatin in living cells.