Community structure of methanogenic archaea in paddy field soil under double cropping (rice-wheat)
Community structure of methanogenic archaea in paddy field soil under double cropping (rice-wheat)
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DOI：
10.1016/j.soilbio.2005.09.020
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发表时间：
2006-06
影响因子：
9.7
通讯作者：
Takeshi Watanabe;M. Kimura;S. Asakawa
中科院分区：
文献类型：
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作者：
Takeshi Watanabe;M. Kimura;S. Asakawa
双季稻田土壤产甲烷古菌群落结构研究和小麦[Triticum aestivum L.]）采用变性梯度凝胶电泳（DGGE）方法对该菌进行了研究。采用DGGE技术，对两个稻田在淹水和高地条件下的土壤样品进行了7次和6次的分析，并利用0357 F-GC/0691 R和1106 F-GC/1378 R两对引物进行了分析。用引物0357 F-GC/0691 R和1106 F-GC/1378 R在DGGE凝胶上分别观察到25和29条不同的条带。DGGE带型的产甲烷古菌群落是稳定的，全年包括水稻在淹水条件下和小麦在高地条件下的培养期间。聚类分析和主成分分析结果表明，土壤类型（采样区域）的差异对稻田土壤产甲烷古菌群落结构影响较大，而采样时期和不同施肥处理对产甲烷古菌群落结构影响较小。从DGGE条带中获得的大部分序列与甲烷微生物目、甲烷微藻科、甲烷小球藻科和水稻类群I密切相关。
Community structure of methanogenic archaea in paddy field soil under double cropping (rice [Oryza sativa L.] and wheat [Triticum aestivum L.]) was studied by the denaturing gradient gel electrophoresis (DGGE) method. Soil samples under flooded and upland conditions were collected 7 and 6 times, respectively, from two paddy fields throughout a year, and two primer sets, 0357F-GC/0691R and newly designed 1106F-GC/1378R, were used for DGGE analysis. The 25 and 29 different bands were observed on the DGGE gels with the primers 0357F-GC/0691R and 1106F-GC/1378R, respectively. DGGE band patterns of the methanogenic archaeal community were stable throughout a year including the cultivation periods of rice under flooded conditions and of wheat under upland conditions. Cluster analysis and principal component analysis suggested that the difference in the soil type (sampling region) largely influenced the community structures of methanogenic archaea in paddy field soil, while the effects of sampling period and different fertilizer treatments on them were small. Most of the sequences obtained from the DGGE bands were closely related to Methanomicrobiales, Methanosarcinaceae, Methanosaetaceae and Rice cluster-I.