Comparison of protein adsorption isotherms and uptake rates in preparative cation-exchange materials
Comparison of protein adsorption isotherms and uptake rates in preparative cation-exchange materials
复制标题
DOI：
10.1016/s0021-9673(98)00796-1
复制
发表时间：
1998-12-11
影响因子：
4.1
通讯作者：
Lenhoff, AM
中科院分区：
文献类型：
--
作者：
Chang, C;Lenhoff, AM
本文研究了溶菌酶在六种制备型阳离子交换固定相上的吸附等温线和有效扩散系数。孔扩散和均相扩散模型被用于估计扩散系数，等温线拟合到非朗缪尔分析等温线方程。从等温线推断的能力被认为是与溶菌酶的表面积，获得的有效表面浓度与在各种非色谱系统中通过不同的方法测量的值一致。孔扩散系数随蛋白质和盐浓度的变化呈现出系统的趋势，孔尺寸和连通性的影响也很明显。在均匀扩散系数的一些趋势是完全不同的那些在孔隙扩散系数，但这些差异基本上消失时，均匀扩散系数重新调整，以考虑吸附平衡行为。需要更多的信息，以进一步阐明耦合扩散和吸附在固定相的机制。(C)1998 Elsevier Science B.V.保留所有权利。
Adsorption isotherms and effective diffusivities of lysozyme in a set of six preparative cation-exchange stationary phases were determined from batch uptake data in a stirred vessel. Both a pore diffusion and a homogeneous diffusion model were used in estimating diffusivities, with the isotherms fitted to a non-Langmuirian analytical isotherm equation. The capacities inferred from the isotherms are found to be correlated with the surface area accessible to lysozyme, the effective surface concentrations obtained being in agreement with values measured by different methods in various non-chromatographic systems. The pore diffusivities show systematic trends with protein and salt concentration, and effects of pore size and connectivity are also evident. Some trends in the homogeneous diffusivities are quite different to those in the pore diffusivities, but these differences largely disappear when the homogeneous diffusivities are rescaled to account for adsorption equilibrium behavior. Additional information is required to elucidate further the mechanisms of coupled diffusion and adsorption in stationary phases. (C) 1998 Elsevier Science B.V. All rights reserved.