INTERLEUKIN-1 beta INDUCES SLEEP INDEPENDENT OF PROSTAGLANDIN D-2 IN RATS AND MICE
INTERLEUKIN-1 beta INDUCES SLEEP INDEPENDENT OF PROSTAGLANDIN D-2 IN RATS AND MICE
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Interleukin-1beta 在大鼠和小鼠中诱导睡眠，不依赖于前列腺素 D2。
DOI：
10.1016/j.neuroscience.2016.09.053
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发表时间：
2017
期刊：
影响因子：
3.3
通讯作者：
Qu Wei-Min
中科院分区：
文献类型：
--
作者：
Zhang Bin-Jia;Shag Shu-Rong;Aritake Kosuke;Takeuchi Atsuko;Urade Yoshihiro;Huang Zhi-Li;Lazarus Michael;Qu Wei-Min
白介素1β(IL-1β)和前列腺素D2(PG D2)是内源性促睡眠物质。据报道，一种高选择性的环氧合酶-2(COX-2)抑制剂NS398能完全抑制IL-1β诱导的大鼠睡眠，IL-1β诱导的睡眠被认为是由前列腺素D2介导的。然而，在本研究中，在23：00至5：00的夜间(活跃期)，将IL-1β(10 Ng)注射至大鼠基底前脑对PGD2敏感的睡眠促进区的蛛网膜下腔，注射NS398(3 mg/kg)并不能抑制64.2%增加的非快速眼动(非快速眼动，NREM)睡眠。同时，IL-1β剂量为1.7g/kg和5μg/kg时，野生型(WT)小鼠在20：00(点燃时间)注射后6h的非快速眼动睡眠时间分别增加76.8%和121.1%，野生型(WT)小鼠分别增加67.7%和147.3%，PGD2受体(DP1R)基因敲除小鼠分别增加108.9%和121.6%。这些结果表明，IL-1β诱导的大鼠非快眼动睡眠不依赖于PGD2/DP1R系统和其他COX-2衍生的前列腺素。
Interleukin-1β (IL-1β) and prostaglandin (PG) D2are endogenous sleep-promoting substances. Since it was reported that a highly selective cyclooxygenase-2 (COX-2) inhibitor, NS398, completely inhibited IL-1β-induced sleep in rats, IL-1β-induced sleep had been believed to be mediated by prostanoids, most probably PGD2. However, in the present study, pretreatment of rats with NS398 (3 mg/kg) did not suppress the 64.2% increased non-rapid eye movement (non-REM, NREM) sleep during infusion of IL-1β (10 ng) for 6 h in the nocturnal (active) period between 23:00 and 5:00 into the subarachnoid space of the PGD2-sensitive sleep-promoting zone of the basal forebrain. Meanwhile, IL-1β at doses of 1.7 and 5 μg/kg also significantly increased NREM sleep for 6 h after intraperitoneal injection at 20:00 (light-off time) by 76.8% and 121.1%, respectively, in wild-type (WT) mice, by 67.7% and 147.3%, respectively, in WT mice pretreated with NS398 (5 mg/kg) and by 108.9% and 121.6%, respectively, in PGD2receptor (DP1R) knockout mice. These results indicate that IL-1β-induced NREM sleep is independent of the PGD2/DP1R system and other COX-2-derived prostaglandins in rats and mice.