Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain.
Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain.
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DOI：
10.1007/s00705-023-05951-y
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发表时间：
2024-01
影响因子：
2.7
通讯作者：
Hao Wang;Yongfang Mo;Wenbo Liu;Chenxia Niu;Qijie He;Tongwei Ren;Ouyang Kang;Ying Chen;Weijian Huang;Zuzhang Wei
中科院分区：
文献类型：
--
作者：
Hao Wang;Yongfang Mo;Wenbo Liu;Chenxia Niu;Qijie He;Tongwei Ren;Ouyang Kang;Ying Chen;Weijian Huang;Zuzhang Wei
塞内卡病毒A （SVA）是一种引起猪水疱病的新兴病毒。构建一个完整的SVA cDNA克隆是了解其复制和发病机制的关键。本研究成功构建了SVA/GX/CH/2018的cmv启动子驱动感染性cDNA克隆，命名为rSVA GX01。pSVA GX01与亲本分离株（SVA/GX/CH/2018）的序列比较发现3个单核苷酸差异。实验用4周龄仔猪感染亲本病毒或克隆病毒。结果表明，与亲本病毒SVA CH-GX-01-2018相比，克隆的rSVA GX01对4周龄猪的致病性较弱。SVA的感染克隆将为研究病毒复制周期和病毒基因组功能分析提供有价值的工具。
Senecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pathogenicity in 4-week-old pigs compared to the parental virus SVA CH-GX-01-2018. The infectious clone of SVA will serve as a valuable tool for studying the viral replication cycle and for functional analysis of the viral genome.