Serological evidence of Merkel cell polyomavirus primary infections in childhood
Serological evidence of Merkel cell polyomavirus primary infections in childhood
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DOI：
10.1016/j.jcv.2010.10.015
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发表时间：
2011-02-01
影响因子：
8.8
通讯作者：
Hedman, Klaus
中科院分区：
文献类型：
--
作者：
Chen, Tingting;Hedman, Lea;Hedman, Klaus
背景：默克尔细胞多瘤病毒（MCPyV）是2008年新发现的一种病毒，被认为是默克尔细胞癌（MCC）的致病因子。最近的分子和血清学数据表明，MCPyV感染是常见的在一般population.Objectives：本研究的目的是调查的年龄主要暴露于MCPyV.Study design：一个MCPyV-IgG EIA开发使用的MCPyV主要衣壳蛋白VP 1表达和自组装成病毒样颗粒（VLP）在昆虫细胞。用该方法检测两组儿童血清IgG抗体。第1组包括来自217名患有急性下呼吸道感染的儿童（3-13岁）的配对和5-8年随访血清。第2组包括158名儿童（1-4岁）中耳炎的血清; 86名儿童接受腺样体切除术，72名未接受腺样体切除术，3年后获得随访血清。结果：第1组受试者1-4岁时MCPyV-IgG的患病率为9%，4-13岁时增加到35%，5-8年期间血清转换率为33%。在第2组中，3年期间的血清转换率为16%。结论：MCPyV原发性感染在儿童期普遍存在，首次暴露年龄较轻。血清学结果显示，没有证据表明MCPyV在下呼吸道感染中具有致病作用，表现为急性喘息，但与扁桃体中MCPyV持续存在的概念一致。(C)2010年爱思唯尔B。V.保留所有权利。
Background: Merkel cell polyomavirus (MCPyV) was identified newly (2008) and is believed to be an etiologic factor of Merkel cell carcinoma (MCC). Recent molecular and serological data suggest that MCPyV infection is common in the general population.Objectives: The aim of this study was to investigate the age of primary exposure to MCPyV.Study design: A MCPyV-IgG EIA was developed using the MCPyV major capsid protein VP1 expressed and self-assembled into virus-like particles (VLPs) in insect cells. The assay was used to detect serum IgG antibodies in two groups of children. Group 1 comprised paired and 5-8 year follow-up sera from 217 children (3-13 years) with acute lower respiratory tract infection. Group 2 comprised sera from 158 children (1-4 years) with otitis media; 86 children underwent adenoidectomy and 72 did not, whereafter follow-up sera were obtained 3 years later.Result: The prevalence of MCPyV-IgG was 9% at 1-4 years, and increased to 35% at 4-13 years among subjects from Group 1, with a 33% seroconversion rate during 5-8 years. Among Group 2, the seroconversion rate was 16% during 3 years. The IgG prevalence at 4-7 years as well as the IgG levels showed an apparent gender difference, with male preponderance prevailing among the children without adenoidectomy.Conclusion: MCPyV primary infections occur ubiquitously in childhood, and the first exposure takes place at young age. The serology showed no evidence for a causative role of MCPyV in lower respiratory tract infection manifesting as acute wheezing, but was compatible with the notion of MCPyV persistence in tonsils. (C) 2010 Elsevier B. V. All rights reserved.