Analysis of ras gene mutations in biliary and pancreatic tumors by polymerase chain reaction and direct sequencing
Analysis of ras gene mutations in biliary and pancreatic tumors by polymerase chain reaction and direct sequencing
复制标题
聚合酶链反应和直接测序分析胆胰肿瘤ras基因突变
作者：
M. Tada;O. Yokosuka;M. Omata;M. Ohto;K. Isono
Ras基因是人类癌症中最常检测到的一种癌基因，由三个家族（H-ras、K-ras、N-ras）组成，它们通过密码子12、13或61处的点突变转化为活性癌基因。作者分析了12例肝外胆管癌，9例胆囊癌，20例胰腺肿瘤（18例胰腺腺癌和2例胰岛细胞瘤）中这些密码子的突变，采用聚合酶链反应和直接测序方法直接测序核苷酸。在所有18例胰腺癌和1例胆管癌中发现了K-ras密码子12的点突变，但在其余11例胆管癌、所有9例胆囊癌或2例胰岛细胞瘤中没有发现突变。ras基因突变的发生率很高，可用于临床诊断胰腺癌的争议案件。
Ras gene is one of the oncegenes most commonly detected in human cancers and consists of three families (H‐ras, K‐ras, N‐ras) that are converted to active oncogenes by point mutations occurring in codon 12, 13, or 61. The authors analyzed mutations of these codons in 12 extrahepatic bile duct carcinomas, nine gallbladder carcinomas, and 20 pancreatic tumors (18 pancreatic adenocarcinomas and two islet cell tumors) by a method to directly sequence nucleotides, using polymerase chain reaction and a direct sequencing method. Point mutations at K‐ras codon 12 were found in all of 18 pancreatic adenocarcinomas and in one bile duct carcinoma, but there were no mutations in the remaining 11 bile duct carcinomas, in all of 9 gallbladder carcinomas, or in two islet cell tumors. A very high incidence of ras gene mutations may be used clinically for the diagnosis of debatable cases of pancreatic adenocarcinoma.
影响因子：
16.6
作者：
M. Barbacid
通讯作者：
M. Barbacid
DOI：
10.1073/pnas.86.7.2403
发表时间：
1989-04-01
影响因子：
11.1
作者：
BURMER, GC;LOEB, LA
通讯作者：
LOEB, LA