Molecular cloning and characterization of TRPVs in two rice pests: Nilaparvata lugens (Stal) and Nephotettix cincticeps (Uhler)
Molecular cloning and characterization of TRPVs in two rice pests: Nilaparvata lugens (Stal) and Nephotettix cincticeps (Uhler)
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两种水稻害虫 TRPV 的分子克隆和表征：褐飞虱 (Stal) 和黑尾叶蝉 (Uhler)
DOI：
10.1002/ps.5254
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发表时间：
2019
影响因子：
4.1
通讯作者：
Huang Jia
中科院分区：
文献类型：
--
作者：
Mao Fen;Guo Lei;Jin Miao;Qiao Xiao mu;Ye Gong yin;Huang Jia
背景昆虫TRPV是瞬时受体电位(TRP)离子通道的一个亚家族，包括两个基因，nanchung和inactive。最近发现两种商业杀虫剂吡蚜酮和吡氟喹唑靶向异聚TRPV离子通道复合物，该复合物在果蝇脊索音器官神经元中特异性表达。然而，有关农业昆虫 TRPV 基因的信息仍然有限。 结果在本研究中，我们从亚洲两种严重的水稻害虫褐飞虱 (NlNan 和 NLIav) 和黑尾叶蝉 (NcNan 和 NcIav) 中克隆并鉴定了两个 TRPV 基因。推导的氨基酸序列与其他昆虫同源物具有高度同一性（58-85%），并具有特有的 TRPV 结构域结构：五个锚蛋白重复序列和六个跨膜结构域。这些 TRPV 转录本在所有发育阶段都有表达，并且雄性成虫中的表达水平显着高于雌性成虫。此外，触角中的表达水平远高于头部和腿部。结论NlNan、NlIav、NcNan和NcIav可能在雄性特异性行为中发挥作用，序列信息为进一步研究TRPVs在农业害虫中的结构和功能表征奠定了基础。 © 2018 中国化学工业学会
BACKGROUDInsect TRPV is a subfamily of transient receptor potential (TRP) ion channels, including two genes,nanchungandinactive. It has recently been found that two commercial insecticides, pymetrozine and pyrifluquinazon, target the heteromeric TRPV ion channel complex which is specifically expressed in the chordotonal organ neurons inDrosophila. However, information on the TRPV genes in agricultural insects is still limited.RESULTSIn this study, we cloned and characterized two TRPV genes fromNilaparvata lugens(NlNanandNLIav) andNephotettix cincticeps(NcNanandNcIav), two serious rice pests throughout Asia. The deduced amino acid sequences share highly identity with other insect homologues (58–85%) and have the characteristic TRPV domain architecture: five ankyrin repeats and six transmembrane domains. These TRPV transcripts were expressed in all developmental stages and expression levels in male adults were significantly higher than in female adults. Moreover, expression levels in antennae were much higher than in heads and legs.CONCLUSIONNlNan, NlIav, NcNanandNcIavmay have roles in male‐specific behaviors, and the sequence information lays the foundation for further study on the structural and functional characterization of TRPVs in agricultural pests. © 2018 Society of Chemical Industry