Polymalic acid fermentation by Aureobasidium pullulans for malic acid production from soybean hull and soy molasses: Fermentation kinetics and economic analysis
Polymalic acid fermentation by Aureobasidium pullulans for malic acid production from soybean hull and soy molasses: Fermentation kinetics and economic analysis
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DOI：
10.1016/j.biortech.2016.10.042
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发表时间：
2017-01-01
影响因子：
11.4
通讯作者：
Yang, Shang-Tian
中科院分区：
文献类型：
--
作者：
Cheng, Chi;Zhou, Yipin;Yang, Shang-Tian
出芽短梗霉ZX-10以豆壳水解液和玉米浆为原料生产多聚苹果酸，苹果酸产量接近0.4g/g，产率接近0.5g/L。ZX-10还能发酵大豆糖蜜，将包括棉子糖家族低聚糖在内的所有碳水化合物转化为多马来酸，在氮气限制下的补料分批发酵中，苹果酸的效价高达71.9g/L，产率为0.69g/L·h。较高的生产力为0.64g/L。通过细胞循环和补加CSL的重复分批发酵获得H。对5000公吨工厂的成本分析表明，从大豆糖蜜中生产苹果酸的价格为1.10美元/公斤，从玉米中生产苹果酸的价格为1.37美元/公斤，从豆壳中生产苹果酸的价格为1.74美元/公斤。以1.75美元/公斤的市场价格，通过PMA发酵从大豆糖蜜中生产苹果酸，为工业化生产生物基苹果酸提供了一种具有经济竞争力的工艺。(C)2016爱思唯尔有限公司。保留所有权利。
Polymalic acid (PMA) production by Aureobasidium pullulans ZX-10 from soybean hull hydrolysate supplemented with corn steep liquor (CSL) gave a malic acid yield of similar to 0.4 g/g at a productivity of similar to 0.5 g/L.h ZX-10 can also ferment soy molasses, converting all carbohydrates including the raffinose family oligosaccharides to PMA, giving a high titer ( 71.9 g/L) and yield ( 0.69 g/g) at a productivity of 0.29 g/L.h in fed-batch fermentation under nitrogen limitation. A higher productivity of 0.64 g/L . h was obtained in repeated batch fermentation with cell recycle and CSL supplementation. Cost analysis for a 5000 MT plant shows that malic acid can be produced at $ 1.10/kg from soy molasses, $ 1.37/kg from corn, and $ 1.74/kg from soybean hull. At the market price of $ 1.75/kg, malic acid production from soy molasses via PMA fermentation offers an economically competitive process for industrial production of bio-based malic acid. (C) 2016 Elsevier Ltd. All rights reserved.