Cancer Exosomes as Mediators of Drug Resistance
Cancer Exosomes as Mediators of Drug Resistance
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DOI：
10.1007/978-1-4939-3347-1_13
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Lyden, David
中科院分区：
文献类型：
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作者：
Andre, Maria do Rosario;Pedro, Ana;Lyden, David
在过去几十年中，多项研究表明，肿瘤微环境不仅是肿瘤进展和转移的关键决定因素，也是对治疗产生耐药性的关键决定因素。外泌体是一种内吞起源的小膜泡，包含mRNA、DNA片段和蛋白质，由许多不同的细胞类型释放，包括癌细胞。越来越多的证据表明，癌症来源的外泌体有助于肿瘤微环境相关成分的募集和重编程。了解外泌体和肿瘤微环境如何影响耐药性，将有助于制定新的、更好的策略来克服耐药性并治疗癌症。在此，我们描述了一种从细胞培养物、新鲜和冷冻血浆中纯化外泌体的技术，并通过电子显微镜、纳米颗粒跟踪分析显微镜（NanoSight microscope）和蛋白质印迹法（Western blot）进行进一步分析。
In the last decades, several studies demonstrated that the tumor microenvironment is a critical determinant not only of tumor progression and metastasis, but also of resistance to therapy. Exosomes are small membrane vesicles of endocytic origin, which contain mRNAs, DNA fragments, and proteins, and are released by many different cell types, including cancer cells. Mounting evidence has shown that cancer-derived exosomes contribute to the recruitment and reprogramming of constituents associated with the tumor microenvironment. Understanding how exosomes and the tumor microenvironment impact drug resistance will allow novel and better strategies to overcome drug resistance and treat cancer.Here, we describe a technique for exosome purification from cell culture, and fresh and frozen plasma, and further analysis by electron microscopy, NanoSight microscope, and Western blot.