Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles.
Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles.
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DOI：
10.1152/physiolgenomics.00051.2008
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发表时间：
2009-03
影响因子：
4.6
通讯作者：
E. C. Pacheco-Pinedo;M. Budak;U. Zeiger;L. Jørgensen;S. Bogdanovich;H. Schrøder;N. Rubinstein;T. Khurana
中科院分区：
文献类型：
--
作者：
E. C. Pacheco-Pinedo;M. Budak;U. Zeiger;L. Jørgensen;S. Bogdanovich;H. Schrøder;N. Rubinstein;T. Khurana
眼外肌（EOM）是一个独特的肌肉群，显示出一系列独特的收缩，结构和再生特性。它们对某些疾病也有不同的敏感性，并且在杜氏肌营养不良症（DMD）中神秘地幸免。眼外肌与其他骨骼肌是如此不同，以至于术语“同种异型”被创造出来以突出眼外肌组特异性的特性。我们推测，增加和独特的干细胞可能是眼外肌中持续的肌生成的基础。分离并研究侧群（SP）干细胞。眼外肌的SP细胞含量比肢体肌肉高15倍。表达谱分析揭示了348个定义EOM-SP转录组的转录本。超过92%的转录物是SP特异性的，因为它们在以前的全肌肉微阵列研究中不存在。培养的EOM-SP细胞显示出上级体外增殖能力。最后，对从眼外肌和肢体肌肉分离的肌纤维进行的定向祖细胞或卫星细胞的测定独立地验证了这些肌肉的增殖能力增加。我们提出了一种模型，其中独特的眼外肌干细胞有助于眼外肌中注意到的持续肌生成，并与其在DMD中的作用一致。我们相信，更多数量的干细胞，它们独特的转录组，更大的增殖能力的眼外肌干细胞，以及更多数量的卫星细胞也提供了线索，为新的基于细胞的治疗策略。
The extraocular muscles (EOMs) are a distinct muscle group that displays an array of unique contractile, structural, and regenerative properties. They also have differential sensitivity to certain diseases and are enigmatically spared in Duchenne muscular dystrophy (DMD). The EOMs are so distinct from other skeletal muscles that the term "allotype" has been coined to highlight EOM group-specific properties. We hypothesized that increased and distinct stem cells may underlie the continual myogenesis noted in EOM. The side population (SP) stem cells were isolated and studied. EOMs had 15x higher SP cell content compared with limb muscles. Expression profiling revealed 348 transcripts that define the EOM-SP transcriptome. Over 92% of transcripts were SP specific, because they were absent in previous whole muscle microarray studies. Cultured EOM-SP cells revealed superior in vitro proliferative capacity. Finally, assays of the committed progenitors or satellite cells performed on myofibers isolated from EOM and limb muscles independently validated the increased proliferative capacity of these muscles. We suggest a model in which unique EOM stem cells contribute to the continual myogenesis noted in EOM and consistent with a role for their sparing in DMD. We believe the greater numbers of stem cells, their unique transcriptome, the greater proliferative capacity of EOM stem cells, and the greater number of satellite cells also offer clues for novel cell-based therapeutic strategies.