Yeast STE7, STE11, and STE12 genes are required for expression of cell-type-specific genes.
Yeast STE7, STE11, and STE12 genes are required for expression of cell-type-specific genes.
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酵母 STE7、STE11 和 STE12 基因是细胞类型特异性基因表达所必需的。
DOI：
10.1128/mcb.8.2.551-556.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
SpragueJr,GF
中科院分区：
文献类型：
--
作者：
Fields,S;Chaleff,DT;SpragueJr,GF
酵母单倍体中的细胞类型特化涉及两组基因（α-特异性基因和α-特异性基因）之一的互斥表达。我们证明了STE 7、STE 11和STE 12基因的产物是两个基因组表达所必需的。从这些基因组转录的RNA水平显着降低，但没有取消在theSTE 7，STE 11，或STE 12基因中含有无效突变的单倍体。在所有三个STE基因中携带突变的菌株中，来自α和α特异性基因组的转录水平没有进一步降低，这表明STE 7、STE 11和STE 12是转录的相同方面所必需的。我们进一步表明，对于特定基因组的每个成员，对这些产品的需求是不一样的。然而，对于任何给定的α-或α-特异性基因，三种突变对RNA水平的影响是相似的。
Cell type specialization in yeast haploids involves the mutually exclusive expression of one of two sets of genes, the a-specific and α-specific genes. We demonstrated that the products of theSTE7,STE11, andSTE12genes were required for the expression of both gene sets. RNA levels transcribed from these gene sets were significantly decreased but not abolished in haploids containing a null mutation in theSTE7,STE11, orSTE12gene. Transcript levels from the a- and α-specific gene sets were not further reduced in strains harboring mutations in all three STE genes, suggesting thatSTE7,STE11, andSTE12are required for the same aspect of transcription. We further showed that the requirement for these products was not the same for each member of a particular gene set. However, for any given a- or α-specific gene, the effect on RNA levels of any of the threestemutations was similar.