Tenfibgen ligand nanoencapsulation delivers bi-functional anti-CK2 RNAi oligomer to key sites for prostate cancer targeting using human xenograft tumors in mice.
Tenfibgen ligand nanoencapsulation delivers bi-functional anti-CK2 RNAi oligomer to key sites for prostate cancer targeting using human xenograft tumors in mice.
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DOI：
10.1371/journal.pone.0109970
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ahmed K
中科院分区：
文献类型：
--
作者：
Trembley JH;Unger GM;Korman VL;Abedin MJ;Nacusi LP;Vogel RI;Slaton JW;Kren BT;Ahmed K
将核酸保护性和特异性递送至恶性细胞仍然是癌症治疗的高度期望的方法。在这里，我们提供了关于Tenorigen（TBG）-壳纳米胶囊技术的物理和化学特性，作用机制和初步治疗效果的数据，用于肿瘤定向递送靶向CK 2 αα'的单链DNA/RNA嵌合寡聚体至小鼠异种移植肿瘤。亚50 nm尺寸的TBG纳米胶囊（s50-TBG）是15 - 20 nm尺寸的略微带负电荷的均匀颗粒，其赋予对核酸货物的保护。DNA/RNA嵌合寡聚体（RNAi-CK 2）通过siRNA和反义机制来降低CK 2 αα'表达水平。s50-TBG-RNAi-CK 2的全身递送特异性靶向恶性细胞，包括骨中的肿瘤细胞，并且在低剂量下降低原位原发性和转移性异种移植前列腺癌肿瘤中的大小和CK 2相关信号。总之，s50-TBG纳米包封技术与靶向CK 2 αα'的嵌合寡聚体一起为前列腺恶性肿瘤的全身治疗提供了显著的前景。
Protected and specific delivery of nucleic acids to malignant cells remains a highly desirable approach for cancer therapy. Here we present data on the physical and chemical characteristics, mechanism of action, and pilot therapeutic efficacy of a tenfibgen (TBG)-shell nanocapsule technology for tumor-directed delivery of single stranded DNA/RNA chimeric oligomers targeting CK2αα' to xenograft tumors in mice. The sub-50 nm size TBG nanocapsule (s50-TBG) is a slightly negatively charged, uniform particle of 15 - 20 nm size which confers protection to the nucleic acid cargo. The DNA/RNA chimeric oligomer (RNAi-CK2) functions to decrease CK2αα' expression levels via both siRNA and antisense mechanisms. Systemic delivery of s50-TBG-RNAi-CK2 specifically targets malignant cells, including tumor cells in bone, and at low doses reduces size and CK2-related signals in orthotopic primary and metastatic xenograft prostate cancer tumors. In conclusion, the s50-TBG nanoencapsulation technology together with the chimeric oligomer targeting CK2αα' offer significant promise for systemic treatment of prostate malignancy.
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影响因子：
46.9
作者：
Dassie, Justin P.;Liu, Xiu-ying;Thomas, Gregory S.;Whitaker, Ryan M.;Thiel, Kristina W.;Stockdale, Katie R.;Meyerholz, David K.;McCaffrey, Anton P.;McNamara, James O., II;Giangrande, Paloma H.
通讯作者：
Giangrande, Paloma H.
寡核苷酸药物中的靶向mRNA抑制。
影响因子：
14.9
作者：
Lightfoot HL;Hall J
通讯作者：
Hall J
影响因子：
8.4
作者：
Giusiano, Sophie;Cachet, Claude;Charpin, Colette
通讯作者：
Charpin, Colette
影响因子：
5.7
作者：
GAPANY, M;FAUST, RA;AHMED, K
通讯作者：
AHMED, K
影响因子：
5.3
作者：
Buchou, T;Vernet, M;Boldyreff, B
通讯作者：
Boldyreff, B