Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology
Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology
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DOI：
10.1016/j.coisb.2021.100373
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发表时间：
2021-12-01
影响因子：
3.7
通讯作者：
Noireaux, Vincent
中科院分区：
文献类型：
--
作者：
Garenne, David;Bowden, Steven;Noireaux, Vincent
近十年来，无细胞基因表达（CFE）的疗效有了很大的提高。因此，CFE系统现在有能力表达由数十个编码复杂自组装过程的基因组成的dna。最近的一个例子证明，传染性噬菌体可以从它们的基因组中通过一锅CFE反应合成。这一具有里程碑意义的结果为从天然或合成DNA生产体外大型生物系统开辟了新的视角。本文综述了单锅CFE反应中病毒的合成和自组装的最新进展，主要涉及噬菌体。我们讨论了在CFE系统中生产病毒样颗粒和噬菌体用于生物医学应用的优势和潜力。由于噬菌体是地球上最丰富、最多样化的生命形式，在试管中动态合成整个或部分噬菌体可以促进发现新的生物功能，并利用其自组装特性用于材料科学应用。
The efficacy of cell-free gene expression (CFE) has been considerably improved in the last decade. As a consequence, CFE systems have now the capacity to express DNAs composed of tens of genes encoding for complex self -assembly processes. A recent example is a demonstration that infectious bacteriophages can be synthesized in one-pot CFE reactions from their genomes. This landmark result opens new perspectives for producing in vitro large biological systems from natural or synthetic DNA. In this article, we review the recent progress in the synthesis and self-assembly of viruses in one-pot CFE reactions, primarily covering bacteriophages. We discuss the advantages and potential of producing virus -like particles and phages in CFE systems for biomedical ap-plications. Because they are the most abundant and diverse life forms on Earth, dynamically synthesizing whole or parts of bacteriophages in test tubes could facilitate uncovering novel biological functions and exploiting their self-assembly proper-ties for material sciences applications.