Morphological organization of oligodendrocyte processes during development in culture and in vivo.
Morphological organization of oligodendrocyte processes during development in culture and in vivo.
复制标题
培养和体内发育过程中少突胶质细胞过程的形态组织。
DOI：
10.1159/000111413
复制
发表时间：
1996
影响因子：
2.9
通讯作者：
Barbarese,E
中科院分区：
文献类型：
--
作者：
Barry,C;Pearson,C;Barbarese,E
为了满足髓鞘生物合成的要求，少突胶质细胞的过程必须分化成专门的细胞区室。为了使髓鞘碱性蛋白mRNA的翻译发生在少突胶质细胞过程或髓鞘膜中，这些结构必须包含核糖体和蛋白质翻译机器的其他组分。光学显微镜和电子显微镜（EM）被用来探索这种可能性，通过分析细胞骨架组织和细胞器人口的过程中的少突胶质细胞在体内和培养的发展。微管（MTs）很少，并捆绑在年轻的少突胶质细胞的培养过程。其中一些过程的特征在于额外存在的蛇形MT。电镜显示，在未成熟和成熟的少突胶质细胞过程中存在的核糖体簇。在前者中，这些簇经常被发现在规则的间隔沿着的过程。类似的间隔特征是髓鞘样图形沿着培养物中成熟细胞的突起分布。无论是在体内还是在培养中，随着细胞的成熟，这些突起变得富含内质网池和线粒体。细胞器的空间排列与髓鞘组装位点处或附近的过程中发生的蛋白质和脂质合成是相容的。
In order to meet the requirements for myelin biogenesis, the processes of oligodendrocytes must differentiate into specialized cellular compartments. For translation of myelin basic protein mRNA to occur in the oligodendrocyte processes or the myelin membrane, these structures must contain ribosomes and other components of the protein translation machinery. Light microscopy and electron microscopy (EM) were used to explore this possibility by analyzing the cytoskeletal organization and the organelle population of the processes of oligodendrocytes during development in vivo and in culture. Microtubules (MTs) were few and bundled in the younger processes of oligodendrocytes in culture. Some of these processes were characterized by the additional presence of serpentine MTs. EM revealed the presence of clusters of ribosomes in both immature and mature oligodendrocyte processes. In the former these clusters were frequently found at regular intervals along the processes. A similar interval characterized the distribution of myelin-like figures along the processes of mature cells in culture. Both in vivo and in culture, the processes became enriched in endoplasmic reticulum cistemae and mitochondria as the cells matured. The spatial arrangement of cellular organelles is compatible with protein and lipid synthesis occurring in the processes, at or near the site of myelin assembly.