DNA damage induced in vivo evaluated with a non-radioactive alkaline elution technique.
DNA damage induced in vivo evaluated with a non-radioactive alkaline elution technique.
复制标题
使用非放射性碱性洗脱技术评估体内诱导的 DNA 损伤。
DOI：
10.1016/s0304-3835(79)80037-3
复制
发表时间：
1979
期刊：
影响因子：
9.7
通讯作者：
W. Kunz
中科院分区：
文献类型：
--
作者：
M. Schwarz;J. Hummel;K. Appel;R. Rickart;W. Kunz
采用改良的碱性过滤洗脱法，研究了NMRI小鼠体内经各种肝癌致癌物和肝肿大药物处理后肝脏DNA的损伤情况。制备肝细胞核并在聚乙烯醇滤膜上裂解。DNA被洗脱，滤器上和洗脱液中剩余的数量都用比色法测量。二甲基亚硝胺（DMN）、二乙基亚硝胺（DEN）、n -亚硝基somorpholine和甲磺酸甲酯（MMS）对DNA的通过有显著的促进作用，而n -乙酰氨基芴（AAF）、4-二甲氨基偶氮苯（DAB）、苯巴比妥、氟烷和ccl4对DNA的通过没有显著的促进作用。讨论了该短期试验的适用性。
A modification of the alkaline filter elution test was used to study damage to liver DNA of NMRI mice following in vivo treatment with various hepatocarcinogens and drugs causing liver enlargement. Liver cell nuclei were prepared and lysed on top of polyvinyl filters. DNA was eluted, and the amounts both remaining on the filter and in the eluate were measured colorimetrically. Dimethylnitrosamine (DMN), diethylnitrosamine (DEN),N-nitrosomorpholine and methyl methanesulfonate (MMS) caused significant enhancement of DNA passage through the filters, whereasN-acetylaminofluorene (AAF), 4-dimethylaminoazobenzene (DAB), phenobarbital, halothane and CCl4did not. The applicability of this short term test is discussed.