CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF ESSENTIAL OIL FROM CURCUMA AERUGINOSA ROXB . RHIZOME

CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF ESSENTIAL OIL FROM CURCUMA AERUGINOSA ROXB . RHIZOME
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铜绿姜黄精油的化学成分和生物活性。

DOI：
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发表时间：
2015
期刊：
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影响因子：
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通讯作者：
C. Kirdmanee
C. Kirdmanee
中科院分区：
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文献类型：
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作者：
O. Theanphong;W. Mingvanish;C. Kirdmanee

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本论文对黄姜黄新鲜根茎的化学成分和生物活性进行了研究。采用气相色谱-质谱联用技术分析了挥发油的化学成分。发现吉马酮是主要成分（23.49%）。考察了挥发油对DPPH自由基、OH自由基、H2 O2自由基的抗氧化活性及还原力。与L-抗坏血酸作为阳性对照相比，发现两种精油对DPPH自由基和OH自由基的清除效果无显著统计学差异（EC 50分别为24.32和244.48 μ g/ml）和L-抗坏血酸（EC_（50）分别为21.21和228.24 μ g/ml），而精油对H_2O_2的清除效果在统计学上显著低于L-抗坏血酸。在还原力测定中，精油没有显示出活性。琼脂扩散法抑菌试验结果表明，挥发油对粪肠球菌（Enterococcus faecalis）ATCC 29212有一定的抑制作用，MIC值为6.25 μ g/ml。四环素（阳性对照）对所有测试细菌均表现出更强的活性，MIC <0.04 μ g/ml。用绿色荧光蛋白微板法（GFPMA）检测其抗结核活性，结果表明其对结核分枝杆菌H37 Ra的MIC值为2500 mg/ml，活性较弱。阳性对照异烟肼的MIC值为0.0230.046 μ g/ml。
This research work has been focused on the elucidation of chemical constituents and biological activities of essential oil hydrodistillated from the fresh rhizome of Curcuma aeruginasa. Chemical constituents of the essential oil were analyzed by GC-MS technique. Germacrone was found to be a major component (23.49%). Antioxidant activities against DPPH  , OH  , H2O2  radicals and reducing power of the essential oil were evaluated. As compared to L-ascorbic acid as a positive control, it was found that there was no significantly statistical difference in the scavenging effectiveness toward DPPH  and OH  radicals between the essential oil (EC50 = 24.32 and 244.48 g/ml, respectively) and L-ascorbic acid (EC50 = 21.21 and 228.24 g/ml, respectively) while the essential oil was statistically significant less effective than L-ascorbic acid in scavenging effectiveness toward H2O2  . For reducing power assay, the essential oil showed no activity. Results of antibacterial activity testing by agar diffusion technique showed that the essential oil has modulate activity against Enterococcus faecalis ATCC 29212 with MIC value of 6.25 g/ml. Tetracyclin, the positive control, exhibited stronger activity against all bacteria tested with MIC  0.04 g/ml. Antimycrobacterial activity of the essential oil examined by green fluorescent protein microplate assay (GFPMA) was weaker activity against Mycobacterium tuberculosis H37Ra (MIC value of 2500 mg/ml). Isoniazid, the positive control, gave the MIC value of 0.0230.046 g/ml.
Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. var. Radiata
DOI： 10.1016/j.foodcont.2007.04.011
发表时间： 2008-04-01
期刊： FOOD CONTROL
影响因子： 6
作者：
Deba, Farah;Xuan, Tran Dang;Tawata, Shinkichi
通讯作者： Tawata, Shinkichi