First Report of Microcystis Strains Producing MC-FR and -WR Toxins in Japan
First Report of Microcystis Strains Producing MC-FR and -WR Toxins in Japan
复制标题
日本首次报道产生 MC-FR 和 -WR 毒素的微囊藻菌株
作者：
Tsuyoshi Ikehara;Kyoko Kuniyoshi;Haruyo Yamaguchi;Yuuhiko Tanabe;T. Sano;M. Yoshimoto;Naomasa Oshiro;Shihoko Nakashima;Mina Yasumoto
微囊藻毒素（Microcystins，MCs）是一类由微囊藻和其他几种蓝藻产生的环状七肽肝毒素。已经使用各种方法表征了许多结构变体，例如液相色谱-质谱（LC-MS）分析、酶联免疫吸附测定（ELISA）和蛋白磷酸酶2A（PP 2A）抑制测定。代表性的MC、MC-LR和相关的蓝藻毒素强烈抑制PP 2A活性，因此可以通过测量PP 2A抑制的程度来测定。然而，这些方法需要用于定量和鉴定已知MC的参考毒素标准。为了获得各种产MC的蓝藻菌株，我们调查和收集产MC的蓝藻，从环境水源在冲绳，日本。使用双重测定（LC-MS分析和PP 2A抑制测定），我们鉴定并分离了产生五种MC变体（MC-LR、-RR、-LA、-FR和-WR）的微囊藻菌株。从微囊藻分离株NIES-4344的实验室培养物中纯化出约4 mg MC-WR和-FR毒素。制备纯的MC-WR和-FR变体以供将来用作LC-MS分析中的毒素标准品。基于ftsZ的系统发育分析表明，NIES-4344菌株属于铜绿微囊藻的鉴定组。这是日本首次报道主要产生MC-WR和-FR毒素的微囊藻菌株。
Microcystins (MCs) are a group of cyclic heptapeptide hepatotoxins produced by Microcystis and several other genera of cyanobacteria. Many structural variants have been characterized using various methods such as liquid chromatography–mass spectrometry (LC-MS) analysis, enzyme-linked immunosorbent assay (ELISA) and protein phosphatase 2A (PP2A) inhibition assay. The representative MC, MC-LR, and related cyanobacterial toxins strongly inhibit PP2A activity and can therefore be assayed by measuring the extent of PP2A inhibition. However, these methods require reference toxin standards for the quantification and identification of known MCs. To obtain various MC-producing cyanobacterial strains, we surveyed and collected MC-producing cyanobacteria from environmental sources of water in Okinawa, Japan. Using a dual assay (LC-MS analysis and PP2A inhibition assay), we identified and isolated Microcystis strains producing five MC variants (MC-LR, -RR, -LA, -FR and -WR). Approximately 4 mg of MC-WR and -FR toxins were purified from the laboratory culture of the Microcystis isolate NIES-4344. Pure MC-WR and -FR variants were prepared for future use as toxin standards in LC-MS analysis. Phylogenetic analysis based on ftsZ revealed that the NIES-4344 strain belongs to the identified groups in Microcystis aeruginosa. This is the first report of Microcystis strains producing mainly MC-WR and -FR toxins in Japan.
DOI：
10.1016/s0021-9258(17)45384-1
发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
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作者：
R. Honkanen;J. Zwiller;R. Moore;S. L. Daily;B. Khatra;M. Dukelow;A. Boynton
通讯作者：
R. Honkanen;J. Zwiller;R. Moore;S. L. Daily;B. Khatra;M. Dukelow;A. Boynton