CONTROL OF SUGAR UTILIZATION IN ORAL STREPTOCOCCI - PROPERTIES OF PHENOTYPICALLY DISTINCT 2-DEOXYGLUCOSE-RESISTANT MUTANTS OF STREPTOCOCCUS-SALIVARIUS
CONTROL OF SUGAR UTILIZATION IN ORAL STREPTOCOCCI - PROPERTIES OF PHENOTYPICALLY DISTINCT 2-DEOXYGLUCOSE-RESISTANT MUTANTS OF STREPTOCOCCUS-SALIVARIUS
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DOI：
10.1111/j.1399-302x.1990.tb00440.x
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发表时间：
1990-12-01
影响因子：
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通讯作者：
VADEBONCOEUR, C
中科院分区：
文献类型：
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作者：
GAUTHIER, L;BOURASSA, S;VADEBONCOEUR, C
报道了通过在乳糖存在下对0.5 mM 2-脱氧葡萄糖耐药的阳性选择分离的唾液链球菌突变体的生理和生化特征。通过一系列的实验，我们发现了两类突变体，包括：生长速率测定，摄取研究，磷酸转移酶系统（PTS）活性的测量和通过蛋白质印迹和[32 P] PEP-磷酸化蛋白的分析检测IIIman蛋白。1类突变体不具有低分子量形式的IIIman。它们不能在甘露糖上生长，也不能转运2-脱氧葡萄糖。另一方面，2类突变体具有2种形式的III man，在甘露糖上容易生长并转运2-脱氧葡萄糖，尽管速率低于亲本菌株。这两类突变体在含有糖的混合物的培养基中表现出异常生长。此外，在所有突变株中观察到编码分解代谢酶的基因的去阻遏。我们的数据表明，甘露糖PTS在控制糖利用的S。唾液肌是复杂的，并且可能涉及几种成分的参与。
The physiological and biochemical characterization of Streptococcus salivarius mutants isolated by positive selection for resistance of 0.5 mM 2-deoxyglucose in the presence of lactose are reported. We found 2 classes of mutants following a series of experiments that included: growth rate determinations, uptake studies, measurement of phosphotransferase system (PTS) activities and detection of the IIIman proteins by Western blotting and analysis of [32P]PEP-phosphorylated proteins. Class 1 mutants did not possess the low-molecular-weight form of IIIman. They did not grow on mannose and were unable to transport 2-deoxyglucose. On the other hand, class 2 mutants possessed the 2 forms of IIIman, grew readily on mannose and transported 2-deoxyglucose, albeit at a lower rate than the parental strain. Both classes of mutants exhibited abnormal growth in media containing mixtures of sugars. Moreover, derepression of genes coding for catabolic enzymes was observed in all the mutant strains. Our data suggested that the role of the mannose PTS in the control of sugar utilization in S. salivarius is complex and may involve the participation of several components.