In Situ Evaluation of Paenibacillus alvei in Reducing Carriage of Salmonella enterica Serovar Newport on Whole Tomato Plants
In Situ Evaluation of Paenibacillus alvei in Reducing Carriage of Salmonella enterica Serovar Newport on Whole Tomato Plants
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DOI：
10.1128/aem.00835-14
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发表时间：
2014-07-01
影响因子：
4.4
通讯作者：
Zheng, Jie
中科院分区：
文献类型：
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作者：
Allard, Sarah;Enurah, Alexander;Zheng, Jie
最近，番茄被认为是肠道沙门氏菌血清型纽波特和其他沙门氏菌血清型食源性爆发的主要媒介。长期的干预措施，以减少沙门氏菌在番茄上的流行仍然难以捉摸的生长和收获后的环境。通过16 S rRNA基因测序鉴定为蜂房类芽孢杆菌的天然存在的细菌是从弗吉尼亚州东海岸番茄种植区的原生植物中附生分离的。在对沙门氏菌和10种与人类食品供应相关的其他细菌病原体进行初步抗微生物活性筛选后，菌株TS-15进一步用于挑战沙门氏菌的减毒株。纽波特对接种的果实，叶，和番茄植物的花朵在昆虫筛选高隧道裂区设计。在第0、1、2和3天以及第5天（花）或第6天（果实和叶），测量接种后具有和不具有拮抗剂的组的沙门氏菌的存活。菌株TS-15对番茄主要食源性病原菌和番茄主要植物病原菌均表现出较强的抑菌活性。在将蜂房青霉菌株TS-15施用于番茄植物的果实、叶和花后，S.纽波特显着下降（P 90%的植物在开花第5天没有可检测到的沙门氏菌水平。天然存在的拮抗剂菌株TS-15在减少沙门氏菌纽波特在整个番茄植物上的携带方面是高度有效的。蜂房青霉TS-15菌株的应用是降低番茄生产中沙门氏菌污染风险的一种有前途的方法。
Recently, tomatoes have been implicated as a primary vehicle in food-borne outbreaks of Salmonella enterica serovar Newport and other Salmonella serovars. Long-term intervention measures to reduce Salmonella prevalence on tomatoes remain elusive for growing and postharvest environments. A naturally occurring bacterium identified by 16S rRNA gene sequencing as Paenibacillus alvei was isolated epiphytically from plants native to the Virginia Eastern Shore tomato-growing region. After initial antimicrobial activity screening against Salmonella and 10 other bacterial pathogens associated with the human food supply, strain TS-15 was further used to challenge an attenuated strain of S. Newport on inoculated fruits, leaves, and blossoms of tomato plants in an insect-screened high tunnel with a split-plot design. Survival of Salmonella after inoculation was measured for groups with and those without the antagonist at days 0, 1, 2, and 3 and either day 5 for blossoms or day 6 for fruits and leaves. Strain TS-15 exhibited broad-range antimicrobial activity against both major food-borne pathogens and major bacterial phytopathogens of tomato. After P. alvei strain TS-15 was applied onto the fruits, leaves, and blossoms of tomato plants, the concentration of S. Newport declined significantly (P 90% of the plants had no detectable levels of Salmonella by day 5 for blossoms. The naturally occurring antagonist strain TS-15 is highly effective in reducing the carriage of Salmonella Newport on whole tomato plants. The application of P. alvei strain TS-15 is a promising approach for reducing the risk of Salmonella contamination during tomato production.