Lipoprotein distribution and biological variation of 24S-and 27-hydroxycholesterol in healthy volunteers
Lipoprotein distribution and biological variation of 24S-and 27-hydroxycholesterol in healthy volunteers
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DOI：
10.1016/j.atherosclerosis.2006.09.026
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发表时间：
2007-09-01
期刊：
影响因子：
5.3
通讯作者：
Rentsch, Katharina M.
中科院分区：
文献类型：
--
作者：
Burkard, Ines;von Eckardstein, Arnold;Rentsch, Katharina M.
24 S-和27-羟基胆固醇是胆固醇代谢的必需中间体，在维持全身胆固醇稳态中起重要作用。采用HPLC-MS法定量测定了健康志愿者脂蛋白亚组分中酯化和未酯化氧化甾醇的分布，以及白天、食物摄入和月经周期对氧化甾醇浓度的影响。此外，在100名健康志愿者中建立了血浆中24 S-和27-羟基胆固醇的参考区间以及HDL扣除中27-羟基胆固醇的相应水平。两种循环氧固醇主要与HDL和LDL一起运输-主要以酯化形式。无论是绝对的或胆固醇相关的血浆水平，没有显着的dumumal变化和月经周期期间没有变化。与血浆中的24 S羟基胆固醇和HDL亚组分中的27-羟基胆固醇相比，男性血浆和非HDL扣除物中27-羟基胆固醇的95%参考区间均高于女性。血浆中27-羟基胆固醇的浓度和非HDL扣除与胆固醇、非HDL胆固醇和甘油三酯的浓度呈强正相关。我们关于氧化固醇脂蛋白分布及其个体内和个体间变异的数据为未来的临床研究奠定了基础。(C)2006爱思唯尔爱尔兰有限公司保留所有权利。
24S- and 27-hydroxycholesterol are obligatory intermediates of cholesterol catabolism and play an important role in the maintenance of whole-body cholesterol homeostasis. Using an HPLC-MS method for oxysterol quantification, the distribution of esterified and unesterified oxysterols in lipoprotein subfractions as well as the influence of daytime, food intake and menstrual cycle on oxysterol concentrations were investigated in healthy volunteers. Moreover, reference intervals for 24S- and 27-hydroxycholesterol in plasma as well as the corresponding levels for 27-hydroxycholesterol in the HDL subtraction were established in 100 healthy volunteers. Both circulating oxysterols are mainly transported in association with HDL and LDL - primarily in the esterified form. No significant diumal changes and no variations during menstrual cycle of either absolute or cholesterol-related plasma levels were detected. In contrast to 24Shydroxycholesterol in plasma and 27-hydroxycholesterol in the HDL subfraction, the 95% reference intervals of 27-hydroxycholesterol both in plasma and the non-HDL subtraction were higher in males than in females. The concentrations of 27-hydroxycholesterol in plasma and the non-HDL subtraction showed strong positive correlations with the concentrations of cholesterol, non-HDL cholesterol and triglycerides. Our data on the lipoprotein distribution of oxysterols as well as on their intra- and inter-individual variation set the stage for future clinical studies. (C) 2006 Elsevier Ireland Ltd. All rights reserved.