Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver β-glucosidase activity
Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver β-glucosidase activity
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DOI：
10.1016/s0014-5793(98)01101-6
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发表时间：
1998-09-25
期刊：
影响因子：
3.5
通讯作者：
Williamson, G
中科院分区：
文献类型：
--
作者：
Day, AJ;DuPont, MS;Williamson, G
黄酮类化合物和黄酮苷类化合物是常见的膳食酚类化合物，可从人的小肠吸收。研究了人小肠和肝脏的无细胞提取物对各种（异）黄酮苷去糖基化的能力。槲皮素4 '-葡萄糖苷、柚皮素7-葡萄糖苷、芹菜素7-葡萄糖苷、染料木素7-葡萄糖苷和大豆苷元7-葡萄糖苷仅被两种组织提取物快速去糖基化，而槲皮素3，4'-二葡萄糖苷、槲皮素3-葡萄糖苷、山奈酚3-葡萄糖苷、槲皮素3-鼠李糖葡萄糖苷和柚皮素7-鼠李糖葡萄糖苷保持不变。槲皮素4 ′-葡萄糖苷和染料木黄酮7-葡萄糖苷在浴组织中水解的Km分别为32 +/- 12和14 +/- 3 μ M。无细胞提取物的酶活性表现出与先前在哺乳动物中描述的胞质广泛特异性β-葡糖苷酶相似的性质。(C)1998年欧洲生物化学学会联合会。
Flavonoid and isoflavonoid glycosides are common dietary phenolics which may be absorbed from the small intestine of humans. The ability of cell-free extracts from human small intestine and liver to deglycosylate various (iso)flavonoid glycosides was investigated. Quercetin 4'-glucoside, naringenin 7-glucoside, apigenin 7-glucoside, genistein 7-glucoside and daidzein 7-glucoside mere rapidly deglycosylated by both tissue extracts, whereas quercetin 3,4'-diglucoside, quercetin 3-glucoside, kaempferol 3-glucoside, quercetin 3-rhamnoglucoside and naringenin 7-rhamnoglucoside remained unchanged. The K-m for hydrolysis of quercetin 4'-glucoside and genistein 7-glucoside was similar to 32 +/- 12 and similar to 14 +/- 3 mu M in bath tissues respectively. The enzymatic activity of the cell-free extracts exhibits similar properties to the cytosolic broad-specificity beta-glucosidase previously described in mammals. (C) 1998 Federation of European Biochemical Societies.