An in vitro study of flow response by cells.
An in vitro study of flow response by cells.
复制标题
细胞流动响应的体外研究。
DOI：
10.1016/0021-9290(71)90013-3
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发表时间：
1971
影响因子：
2.4
通讯作者：
N. Cholvin
中科院分区：
文献类型：
--
作者：
J. Krueger;D. F. Young;N. Cholvin
作为附着的、融合的单层生长的活细胞经受稳定、均匀的层流。细胞在一个特别设计的细胞培养室中生长，在这个培养室中，细胞的流动条件可以精确地定义，并通过实验验证。描述了细胞附近的流动条件，并给出了细胞和亚细胞对观察到的流动条件的响应。在所附几何结构中观察到的细胞响应描述了剪切应力范围从10−3到101dyn/cm2。细胞组分的流变特性的相对重要性随着所施加的剪切应力的大小而变化。这种血流研究的意义在于它们可能用于评估动脉粥样硬化的机械部位偏好。
Living cells grown as an attached, confluent monolayer are subjected to a steady, uniform laminar flow. The cells are grown in a specially designed cell culture chamber in which the flow conditions over the cells may be precisely defined and are experimentally verified.The flow conditions at the cell proximity are described, and the cellular and subcellular responses to the observed flow conditions are presented. The observed responses of the cells in the attached geometry are described for shear stresses ranging from 10−3to 101dyn/cm2. The relative importance of the rheological properties of the cellular components is seen to vary with the magnitude of the shear stress applied.The significance of such flow studies is presented in relation to their possible use in evaulating mechanical site predilection in atherosclerosis.