Nanos suppresses somatic cell fate in Drosophila germ line
Nanos suppresses somatic cell fate in Drosophila germ line
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DOI：
10.1073/pnas.0401647101
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发表时间：
2004-07-13
影响因子：
11.1
通讯作者：
Kobayashi, S
中科院分区：
文献类型：
--
作者：
Hayashi, Y;Hayashi, M;Kobayashi, S
Nanos（Nos）是已知指导生殖系发育的进化保守蛋白之一。在果蝇中，母体Nos蛋白维持生殖系祖细胞或极细胞的转录静止，抑制体细胞基因的异位表达。在这里，我们表明，需要建立和维持生殖系的身份，通过防止细胞凋亡和体细胞的命运。在胚胎发育的中后期，缺乏母体NOS的极细胞因凋亡而退化。当Df（3L）H99抑制细胞凋亡时，一些缺乏Nos的极细胞采用体细胞命运。这些极细胞异位表达体细胞标记，并失去了生殖系标记Vasa。我们进一步发现，一些Nos阴性极细胞能够迁移到性腺，但它们在胚后阶段未能发育为功能性生殖细胞。我们提出了一个模型，在该模型中，一氧化氮建立生殖系/索马二分法，也需要保持生殖系的命运。
Nanos (Nos) is one of the evolutionarily conserved proteins known to direct germ-line development. In Drosophila, maternal Nos protein maintains transcriptional quiescence in the germ-line progenitors or pole cells to repress ectopic expression of somatic genes. Here we show that maternal Nos is required to establish and maintain germ-line identity by preventing apoptosis and somatic cell fate. The pole cells lacking maternal Nos were degraded by apoptosis during mid to late embryogenesis. When apoptosis was suppressed by Df(3L)H99, some pole cells lacking Nos adopted somatic cell fates. These pole cells expressed somatic markers ectopically and lost the germ-line marker Vasa. We further found that some Nos-negative pole cells were able to migrate into the gonads, but they failed to develop as functional germ cells during postembryonic stages. We propose a model in which Nos establishes germ-line/soma dichotomy and is also required to maintain germ-line fate.