Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats
Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats
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DOI：
10.2478/v10136-011-0007-2
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发表时间：
2011
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影响因子：
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通讯作者：
R. Havelek;M. Řezáčová;Z. Sinkorova;L. Zárybnická;J. Pejchal;J. Vávrová
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文献类型：
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作者：
R. Havelek;M. Řezáčová;Z. Sinkorova;L. Zárybnická;J. Pejchal;J. Vávrová
淋巴细胞是对辐射最敏感的细胞之一。在生物体暴露于电离辐射后，它们以与所接收的剂量成比例的速率通过细胞凋亡迅速死亡。因此，它们经常用于生物剂量测定。我们证明，大鼠全身照射后1小时，淋巴细胞核中的组蛋白H2 AX在丝氨酸139上迅速磷酸化，磷酸化过程直接依赖于γ辐射剂量。在这里提出的工作中，我们研究了动力学的淋巴细胞耗竭在外周血中的组蛋白H2 AX的磷酸化外周血淋巴细胞局部（胸部）照射后的大鼠。大鼠全身照射剂量为5戈伊后24小时，淋巴细胞计数下降到几乎为零，而胸部局部照射后，外周血中的淋巴细胞计数保持不变。本文用流式细胞术和显微镜两种方法测定了大鼠胸部照射后1h外周血淋巴细胞γ H_2AX的含量。流式细胞术显示在10-30戈伊剂量范围内γ H2 AX的增加具有剂量依赖性。显微镜方法在较低辐射剂量的情况下更敏感，从低至5戈伊的剂量起，对剂量的依赖性就很明显。该方法能够在5-30戈伊的剂量范围内区分辐射类型，即全身或局部。
Summary Lymphocytes are among the most radiosensitive cells. After exposure of the organism to ionizing radiation, they promptly die by apoptosis at a rate proportional to the dose received. Because of this, they are frequently used in biodosimetry. We demonstrated that one hour after whole-body irradiation of rats, histone H2AX in the lymphocyte nuclei was quickly phosphorylated on serine 139, the phosphorylation process being directly dependent on the gamma radiation dose. In the work presented here, we studied the kinetics of lymphocyte depletion in the peripheral blood and phosphorylation of histone H2AX in the peripheral blood lymphocytes after local (thoracic) irradiation of rats. Twenty-four hours after whole-body irradiation of the rats at a dose of 5 Gy, the lymphocyte count declined to almost zero values, whereas after local irradiation of the thorax area, the counts of lymphocytes in the peripheral blood remained unaltered. The authors employed two methods (flow-cytometric and microscopic) for the γH2AX determination in the peripheral blood lymphocytes, 1 h after thoracic irradiation of rats. Flow cytometry revealed a dose dependence on the increase in γH2AX in a dose range of 10–30 Gy. The microscopic method was more sensitive in the case of lower radiation doses, the dependence on the dose being obvious from a dose as low as 5 Gy. The methods are able, in the dose range 5–30 Gy, to differentiate between the type of irradiation, i.e. the whole-body or local.