A plastid-targeted heat shock cognate 70 kDa protein interacts with the Abutilon mosaic virus movement protein
A plastid-targeted heat shock cognate 70 kDa protein interacts with the Abutilon mosaic virus movement protein
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DOI：
10.1016/j.virol.2010.02.011
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发表时间：
2010-05-25
期刊：
影响因子：
3.7
通讯作者：
Kleinow, Tatjana
中科院分区：
文献类型：
--
作者：
Krenz, Bjoern;Windeisen, Volker;Kleinow, Tatjana
双生病毒的运动蛋白(MP)促进细胞间和植物内的长距离运输，并影响病毒的致病性。酵母双杂交鉴定了拟南芥的一个伴侣蛋白(cpHSC70-1)是阿布蒂龙花叶病毒(AbMV)MP的潜在结合伙伴。在植物中，双分子荧光互补(BIFC)分析显示cpHSC70-1/MP复合体和MP均低聚物位于细胞外围，并与叶绿体共定位。BIFC发现了与叶绿体相关的cPHSC70-1寡聚体，但也分布在细胞边缘和由类似于球茎的质体产生的细丝中。利用AbMV DNA A衍生的基因沉默载体沉默本氏烟草的cpHSC70基因，诱导了微小的白叶面积，这表明这对叶绿体的稳定性有影响。虽然AbMV DNA在褪绿斑点内积累，但这些斑点的空间限制发生了，这表明MP-伴侣相互作用对病毒运输和症状诱导具有功能相关性。(C)2010 Elsevier Inc.保留所有权利。
The movement protein (MP) of bipartite geminiviruses facilitates cell-to-cell as well as long-distance transport within plants and influences viral pathogenicity. Yeast two-hybrid assays identified a chaperone, the nuclear-encoded and plastid-targeted heat shock cognate 70 kDa protein (cpHSC70-1) of Arabidopsis thaliana, as a potential binding partner for the Abutilon mosaic virus (AbMV) MP. In planta, bimolecular fluorescence complementation (BiFC) analysis showed cpHSC70-1/MP complexes and MP homooligomers at the cell periphery and co-localized with chloroplasts. BiFC revealed cpHSC70-1 oligomers associated with chloroplasts, but also distributed at the cellular margin and in filaments arising from plastids reminiscent of stromules. Silencing the cpHSC70 gene of Nicotiana benthamiana using an AbMV DNA A-derived gene silencing vector induced minute white leaf areas, which indicate an effect on chloroplast stability. Although AbMV DNA accumulated within chlorotic spots, a spatial restriction of these occurred, suggesting a functional relevance of the MP-chaperone interaction for viral transport and symptom induction. (C) 2010 Elsevier Inc. All rights reserved.