Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
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DOI：
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发表时间：
1989-09
期刊：
影响因子：
3.3
通讯作者：
A. Ponticelli;Gerald R. Smith
中科院分区：
文献类型：
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作者：
A. Ponticelli;Gerald R. Smith
建立了利用ade6-M26重组热点的突变体筛选方法，用于分离分裂菌pombe减数分裂重组缺陷突变体。9个rec突变是隐性的，定义了6个互补组，并且在纯合时将ade6减数分裂重组减少3倍至大于或等于300倍。进一步分析的三个隐性rec突变也减少了染色体III上ura4上的减数分裂基因内重组和染色体i上pro2和arg3之间的基因间重组。在三个测试间隔中观察到的重组频率的非协调减少表明相应的rec+基因产物具有一定程度的位点特异性（或基因内特异性与基因间特异性）。没有突变特异性灭活ade6-M26热点。其他rec基因可以用这些方法鉴定。
A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than or equal to 300-fold when homozygous. Three recessive rec mutations analyzed further also reduced meiotic intragenic recombination at ura4 on chromosome III and intergenic recombination between pro2 and arg3 on chromosome I. The observed non-co-ordinate reductions of the recombinant frequencies in the three test intervals suggest a degree of locus (or intragenic vs. intergenic) specificity of the corresponding rec+ gene products. None of the mutations specifically inactivated the ade6-M26 hotspot. Additional rec genes may be identified with these methods.