Genetic analysis of the 15;17 chromosome translocation associated with acute promyelocytic leukemia.
Genetic analysis of the 15;17 chromosome translocation associated with acute promyelocytic leukemia.
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与急性早幼粒细胞白血病相关的 15;17 染色体易位的遗传分析。
DOI：
10.1073/pnas.80.16.5007
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发表时间：
1983
影响因子：
11.1
通讯作者：
E. Solomon
中科院分区：
文献类型：
--
作者：
D. Sheer;L. Hiorns;K. Stanley;P. Goodfellow;D. Swallow;S. Povey;N. Heisterkamp;J. Groffen;J. Stephenson;E. Solomon
在胸苷激酶缺陷的小鼠细胞系和急性早幼粒细胞白血病患者的血液白细胞之间构建了体细胞杂交体，该患者表现出与该疾病相关的15 q +; 17 q-染色体易位。一个杂交体含有15 q+易位染色体和很少的其他人类材料。我们已经证明，c-fes癌基因，这已经被定位到15号染色体，是不存在于这个混合，因此，可能是易位到17号染色体。对该杂交种中存在的遗传标记的分析使得能够更精确地定位染色体15和17上的易位断点。我们的实验还实现了对15号和17号染色体上的几个遗传标记的排序和更精确的定位。
Somatic cell hybrids have been constructed between a thymidine kinase-deficient mouse cell line and blood leukocytes from a patient with acute promyelocytic leukemia showing the 15q+;17q- chromosome translocation frequently associated with this disease. One hybrid contains the 15q+ translocation chromosome and very little other human material. We have shown that the c-fes oncogene, which has been mapped to chromosome 15, is not present in this hybrid and, therefore, probably is translocated to the 17q- chromosome. Analysis of the genetic markers present in this hybrid has enabled a more precise localization of the translocation breakpoints on chromosomes 15 and 17. Our experiments also have enabled an ordering and more precise mapping of several genetic markers on chromosomes 15 and 17.