Caenorhabditis elegans reticulon interacts with RME-1 during embryogenesis
Caenorhabditis elegans reticulon interacts with RME-1 during embryogenesis
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DOI：
10.1016/s0006-291x(02)00282-6
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发表时间：
2002-05-03
影响因子：
3.1
通讯作者：
Toyoda, T
中科院分区：
文献类型：
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作者：
Iwahashi, J;Kawasaki, I;Toyoda, T
网状蛋白（RTN）家族蛋白定位于内质网（ER）。在哺乳动物中已经鉴定出至少四种不同的RTN基因。但在大多数情况下除了哺乳动物RTN 4-A和RTN 4-B之外，所编码蛋白的功能是未知的。每个RTN基因通过不同的启动子和选择性剪接产生1-3个蛋白质。在秀丽隐杆线虫中，有一个单一的基因（rtn基因）编码三种网状蛋白，nRTN-A、B和C。nRTN-C的mRNA在生殖细胞和胚胎中表达。然而. nRTN-C蛋白仅在胚胎发生过程中表达，孵化后迅速消失。通过酵母双杂交筛选。分离了编码RME-1（一种在内吞再循环中起作用的蛋白质）的相同C-末端区域的两个克隆。这些发现表明，nRTN-C的功能，在胚胎发生过程中的内吞途径。(C)2002 Elsevier Science（美国）。All rights reserved.
Reticulon (RTN) family proteins are localized in the endoplasmic reticulum (ER). At least four different RTN genes have been identified in mammals. but in most cases. the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are unknown. Each RTN gene produces 1-3 proteins by different promoters and alternative splicing. In Caenorhabditis elegans, there is a single gene (rtn gene) encoding three reticulon proteins, nRTN-A, B, and C. mRNA of nRTN-C is expressed in germ cells and embryos. However. nRTN-C protein is only expressed during embryogenesis and rapidly disappears after hatch. By yeast two-hybrid screening. two clones encoding the same C-terminal region of RME-1, a protein functioning in the endocytic recycling, were isolated. These findings suggest that nRTN-C functions in the endocytic pathway during embryogenesis. (C) 2002 Elsevier Science (USA). All rights reserved.