Protein secretion by the mouse trophoblast during attachment and outgrowth in vitro.
Protein secretion by the mouse trophoblast during attachment and outgrowth in vitro.
复制标题
小鼠滋养层在体外附着和生长过程中的蛋白质分泌。
DOI：
10.1095/biolreprod43.2.251
复制
发表时间：
1990
影响因子：
3.6
通讯作者：
Nieder,GL
中科院分区：
文献类型：
--
作者：
Nieder,GL
蛋白质分泌从小鼠囊胚进行附着和滋养层生长在体外进行了评估。当第5天的囊胚在含血清的培养基中培养时，24小时内就会开始分泌几种“附着相关”蛋白（PAS），与附着和生长同时发生。一旦附着开始，附着前胚泡的这些蛋白质特征就消失了，或者只占分泌物的一小部分。贴壁胚分泌的主要蛋白PA 1是一种分子量为35000 - 45000 M的糖蛋白，具有多种等电形式。PA 2和PA 3分别为40000 M和72000 M的碱性蛋白。PAS的分泌过程中生长的纤连蛋白涂层的塑料在无血清培养基，但不是由胚泡在无血清培养基中的未涂层的塑料在培养过程中保持在非附着状态。在前附着囊胚，分泌的蛋白质产生的滋养层囊泡，但不是由孤立的内细胞团。体外生长的滋养层小泡和通过手术从扩张的囊胚中分离的滋养层都具有与完整囊胚生长物定性相似的分泌蛋白模式。结果表明，滋养层蛋白分泌的发展持续通过生长和巨细胞转化期。这些变化显然依赖于胚泡附着在合适的基质上，但不依赖于任何其他血清的影响。
Protein secretion from mouse blastocysts undergoing attachment and trophoblast outgrowth in vitro was assessed. When Day 5 blastocysts were cultured in serum-containing medium, secretion of several ‘attachment-associated’ proteins (PAS) was initiated within 24 h, coincident with attachment and outgrowth. Those proteins characteristic of the pre-attachment blastocyst disappeared or made-up only a small portion of the secretions once attachment began. The major secreted protein from attached embryos, PA1, is a 35 000–45 000Mracidic glycoprotein with multiple isoelectric forms. PA2, a group of basic 40 000Mrproteins and PA3 a group of 72 000Mrproteins were also produced during outgrowth. PAS were secreted during outgrowth on fibronectin-coated plastic in serum-free medium, but not by blastocysts held in a non-attachment state during culture in serum-free medium on uncoated plastic. In pre-attachment blastocysts, secreted proteins were produced by trophoblast vesicles, but not by isolated inner cell masses. Both trophoblast vesicles growing out in vitro and surgically isolated trophoblast from spreading blastocysts had secreted protein patterns qualitatively similar to those of intact blastocyst outgrowths. The results indicate that development of trophoblast protein secretion continues through the period of outgrowth and giant cell transformation. These changes are apparently dependent on attachment of the blastocyst to a suitable substrate, but not dependent on any other serum influence.