Preparation, partial characterization and bioactivity of water-soluble polysaccharides from boat-fruited sterculia seeds
Preparation, partial characterization and bioactivity of water-soluble polysaccharides from boat-fruited sterculia seeds
复制标题
DOI：
10.1016/j.carbpol.2007.05.010
复制
发表时间：
2007-11-01
影响因子：
11.2
通讯作者：
Gu, Xiaohong
中科院分区：
文献类型：
--
作者：
Wu, Yan;Cui, Steve W.;Gu, Xiaohong
以胖大海为原料，采用热水浸提、乙醇沉淀的方法提取水溶性粗多糖（SP），经阴离子交换层析分离得到中性多糖（NSP）和酸性多糖（ASP）。采用高效体积排阻色谱法（HPSEC）测定了NSP和ASP的分子量、特性粘数和回转半径。NSP富含葡萄糖（85.86%），含有少量半乳糖、阿拉伯糖和木糖。而ASP主要由半乳糖醛酸（40.13%）组成，沿着有鼠李糖、阿拉伯糖、半乳糖以及少量的木糖和葡萄糖，表明其为果胶样多糖。采用二甲苯致小鼠汽车水肿和棉球肉芽肿模型，检测NSP和ASP的生物活性。结果表明，ASP具有较强的抗炎活性，且呈剂量依赖性。目前研究的结果为这种植物作为其抗炎作用的医学补救措施的传统用途提供了科学依据。版权所有（C）2007由爱思唯尔有限公司出版。保留所有权利。
Crude water-soluble polysaccharides (SP) isolated from boat-fruited sterculia seeds by hot water extraction and ethanol precipitation were fractionated into a neutral polysaccharide (NSP) and an acidic one (ASP) by anion-exchange chromatography. The molecular weight, intrinsic viscosity and radius of gyration of NSP and ASP were determined by high performance size exclusion chromatography (HPSEC). NSP was rich in glucose (85.86%), with small amounts of galactose, arabinose and xylose. Whereas ASP consisted mainly of galacturonic acid (40.13%) along with rhamnose, arabinose, galactose, and small amounts of xylose and glucose, indicating a pectin-like polysaccharide which was confirmed by FT-IR spectra. Bioactivity of NSP and ASP was tested using car edema induced by dimethylbenzene and cotton pellet-induced granuloma tissue in murine models. The results showed ASP possessed a potent dose-dependent anti-inflammatory activity. The results from the current study provided a scientific basis for the traditional use of this plant as a medical remedy for its anti-inflammation effects. Crown copyright (C) 2007 Published by Elsevier Ltd. All rights reserved.