STATE OF THE CALCIUM PUMP OF THE SARCOPLASMIC RETICULUM IN COMPENSATORY HYPER FUNCTION AND HYPERTROPHY OF SKELETAL MUSCLE
STATE OF THE CALCIUM PUMP OF THE SARCOPLASMIC RETICULUM IN COMPENSATORY HYPER FUNCTION AND HYPERTROPHY OF SKELETAL MUSCLE
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DOI：
10.1007/bf00802477
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发表时间：
1974-01-01
影响因子：
0.7
通讯作者：
MEERSON F Z
中科院分区：
文献类型：
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作者：
PANCHENKO L F;ALIEV M K;MEERSON F Z
实验在29只体重2.5-3 kg的雄性家兔上进行。在21只家兔中，通过尽可能多地切除腓肠肌腱，造成比目鱼肌代偿性功能亢进和肥大。以同一动物对侧完整肢体的比目鱼肌作为对照。与普通手术的不同之处仅在于不切断腓肠肌肌腱，对8只家兔进行了模拟手术，术后第6天将家兔断头。将7只家兔的完整比目鱼肌和肥大比目鱼肌在恒温箱中75 ℃干燥至恒重，以测定肥大比目鱼肌中干物质的含量。通过改良的Harigaya技术从其他动物的比目鱼肌中分离SPR样品[6]。将重2g的肌肉样品在具有Teflon容器的Waring搅拌器中以12，000 rpm在含有0.15M KCl和0.005M NaHCO 3的40 ml培养基中均质化80秒。将匀浆在8，000 g下离心20 min。将上清液通过几层纱布，并用3.0 KCl处理至终浓度为0.6 M。在加入KCl后，将微粒体在45，000 g下沉降60分钟2 〜 30分钟。微量元素的残留
Experiments were carried out on 29 male rabbits weighing 2.5-3 kg. Compensatory hyperfunction and hypertrophy of the soleus muscle were produced in 21 rabbits by removing as much of the tendon of the synergist muscle, the gastrocnemius, as possible. The soleus muscles of the contralateral intact limbs of the same animals were used as the control. Mock operations differing from the ordinary operations simply in the fact that the tendons of the gastrocnemius muscle were not divided, were carried out on eight rabbits.The rabbits were decapitated on the sixth day after the operation. The intact and hypertrophied soleus muscles of seven rabbits were dried in an incubator at 75~ to constant weight to determine the content of dry substance in the hypertrophied muscle. Samples of SPR were isolated from the seleus muscles of the other animals by a modified Harigaya's technique [6]. Samples of muscles weighing 2 g were homogenized for 80 sec in a Waring blendor with Teflon container at 12,000 rpm in 40 ml medium containing 0.15 M KCI and 0.005 M NaHCO 3. The homogenates were centrifuged at 8,000 g for 20 min. The supernatant was passed through several layers of gauze and treated with 3.0 KCI up to a final concentration of 0.6 M. The microsomes were sedimented at 45,000 g for 60 rain 2 {~-30 rain after addition of the KCI. The residue of micro-