Microthrix parvicella, a filamentous bacterium isolated from activated sludge: cultivation in a chemically defined medium
Microthrix parvicella, a filamentous bacterium isolated from activated sludge: cultivation in a chemically defined medium
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Microthrix parvicella，一种从活性污泥中分离出来的丝状细菌：在化学成分确定的培养基中培养
DOI：
10.1128/aem.46.4.832-839.1983
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发表时间：
1983
影响因子：
4.4
通讯作者：
["H. Slijkhuis
中科院分区：
文献类型：
--
作者：
["H. Slijkhuis
已经测试了大量的培养基用于培养Microthris parvicella，一种经常存在于氧化沟的活性污泥中的丝状微生物。发现该细菌利用油酸（优选为吐温80）作为碳和能量的唯一来源。硫需要以还原形式存在。测试的培养基从复合物到化学成分确定的培养基不等。在含有吐温80（4克/升）、还原氮和还原硫化合物、钙和镁盐、磷酸盐缓冲液、微量元素、硫胺素和氰钴胺的培养基上获得1.3至1.5克/升的生长产量。生长生物体的最佳温度约为25摄氏度，营养培养基的pH值应高于7。
A large number of media have been tested for cultivating Microthris parvicella, a filamentous microorganism often present in the activated sludge of oxidation ditches. The bacterium was found to utilize oleic acid (preferably as Tween 80) as the sole source of carbon and energy. Sulfur is required in the reduced form. The tested media vary from a complex to a chemically defined medium. Growth yields of 1.3 to 1.5 g/liter were obtained on media containing Tween 80 (4 g/liter), reduced nitrogen and reduced sulfur compounds, calcium and magnesium salts, phosphate buffer, trace elements, thiamin, and cyanocobalamin. The optimum temperature for growing the organism is approximately 25 degrees C, and the pH of the nutrient medium should be above 7.