Sulfonated nanoparticles doped electrospun fibers with bioinspired polynorepinephrine sheath for in vivo solid-phase microexreaction of pharmaceuticals in fish and vegetable
Sulfonated nanoparticles doped electrospun fibers with bioinspired polynorepinephrine sheath for in vivo solid-phase microexreaction of pharmaceuticals in fish and vegetable
复制标题
磺化纳米颗粒掺杂电纺纤维，具有仿生聚去甲肾上腺素鞘，用于鱼类和蔬菜中药物的体内固相微萃取
DOI：
10.1016/j.chroma.2016.05.082
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发表时间：
2016
影响因子：
4.1
通讯作者：
Ouyang Gangfeng
中科院分区：
文献类型：
--
作者：
Qiu Junlang;Chen Guosheng;Zhu Fang;Ouyang Gangfeng
在这项研究中，生物相容性共聚物聚乳酸-co-己内酯（PLCL）掺杂磺化γ- al2o3纳米颗粒，用于静电纺丝在不锈钢丝上。去甲肾上腺素（一种存在于神经递质和贻贝粘附蛋白中的儿茶酚胺）的自聚合进一步包裹了电纺丝纤维，以提高表面亲水性并提供光滑的生物界面。研制了不锈钢丝上改性静电纺丝纤维作为新型定制固相微萃取纤维。与聚二甲基硅氧烷（PDMS）纤维相比，这些纤维具有更高的提取效率，特别是对磺胺类化合物的提取效率更高。SPME纤维的相对标准偏差（rsd）为1.98% ~ 9.86%，纤维间的相对标准偏差（rsd）为4.36% ~ 15.6%。此外，这些纤维也被证明是抗生物污染的，适合在体内取样。自制的SPME纤维成功地应用于活鱼和活蔬菜中药物浓度的测定。通过与液体提取法的比较，验证了该方法的准确性和灵敏度，鱼肌肉的检出限为0.16 ~ 5.35 ng/g，蔬菜茎的检出限为0.02 ~ 8.02 ng/g。
In this study, the biocompatible copolymer Poly(lactic acid-co-caprolactone) (PLCL) doped with sulfonated γ-Al2O3nanoparticles was used for electrospun on stainless wires. The electrospun fibers were further sheathed by the self-polymerization of norepinephrine, a catecholamine found both in neurotransmitters and mussel adhesive proteins, to improve the surface hydrophilicity and provide a smooth bio-interface. The modified electrospun fibers on stainless wires were developed as novel custom-made solid-phase microextraction (SPME) fibers. These fibers exhibited much higher extraction efficiency compared to the polydimethylsiloxane (PDMS) fibers, especially to the sulfonamides. The custom-made SPME fibers also showed excellent stability with the relative standard deviations (RSDs) of intra-fiber ranged from 1.98% to 9.86% and RSDs of inter-fiber ranged from 4.36% to 15.6%. Moreover, these fibers were also demonstrated to be anti-biofouling and suitable for in vivo sampling. The custom-made SPME fibers were successfully applied to determine the Pharmaceutical concentrations in living fishes and vegetables. The accuracies were verified by the comparison with liquid extraction and the sensitivities were demonstrated to be satisfying with the limits of detection (LODs) ranged from 0.16 ng/g to 5.35 ng/g in fish muscle and 0.02 ng/g to 8.02 ng/g in vegetable stem.