MicroRNA172 controls inflorescence meristem size through regulation of APETALA2 in Arabidopsis.
MicroRNA172 controls inflorescence meristem size through regulation of APETALA2 in Arabidopsis.
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在拟南芥中，MicroRNA172 通过调节 APETALA2 来控制花序分生组织的大小。
DOI：
10.1111/nph.18111
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Sang Q
中科院分区：
文献类型：
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作者：
Sang Q
APETALA2 (AP2) 转录因子调节拟南芥中的花发育、花转变和茎尖分生组织 (SAM) 维持。AP2 也在转录后水平受到 microRNA172 (miR172) 的调节，但其对 SAM 维持的贡献知之甚少。 miR172。对这些品系和mir172突变体进行表型和遗传分析，以研究miR172对AP2调节对分生组织大小和花产量的作用。我们发现rAP2通过增加细胞大小和细胞数量来扩大花序分生组织。 rAP2从异源启动子的错误表达表明AP2作用于中央区(CZ)和组织中心(OC)以增加SAM大小。此外，我们发现 AP2 受到生长素反应因子 3 (ARF3) 的负向调节。然而，遗传分析表明，ARF3 也独立于 AP2 影响 SAM 大小和成花率。该研究将 miR172/AP2 确定为控制花序分生组织大小的调节模块，并表明 ARF3 对 AP2 的转录调节可微调 SAM 大小的确定。
The APETALA2 (AP2) transcription factor regulates flower development, floral transition and shoot apical meristem (SAM) maintenance in Arabidopsis.AP2is also regulated at the post‐transcriptional level by microRNA172 (miR172), but the contribution of this to SAM maintenance is poorly understood.We generated transgenic plants carrying a form ofAP2that is resistant to miR172 (rAP2) or carrying a wild‐typeAP2susceptible to miR172. Phenotypic and genetic analyses were performed on these lines andmir172mutants to study the role ofAP2regulation by miR172 on meristem size and the rate of flower production.We found thatrAP2 enlarges the inflorescence meristem by increasing cell size and cell number. Misexpression ofrAP2from heterologous promoters showed thatAP2acts in the central zone (CZ) and organizing center (OC) to increase SAM size. Furthermore, we found that AP2 is negatively regulated by AUXIN RESPONSE FACTOR 3 (ARF3). However, genetic analyses indicated that ARF3 also influences SAM size and flower production rate independently of AP2.The study identifies miR172/AP2 as a regulatory module controlling inflorescence meristem size and suggests that transcriptional regulation ofAP2by ARF3 fine‐tunes SAM size determination.