Intrinsic tryptophan fluorescence in the detection and analysis of proteins: a focus on Förster resonance energy transfer techniques.
Intrinsic tryptophan fluorescence in the detection and analysis of proteins: a focus on Förster resonance energy transfer techniques.
复制标题
DOI：
10.3390/ijms151222518
复制
发表时间：
2014-12-05
影响因子：
5.6
通讯作者：
Chung SJ
中科院分区：
文献类型：
--
作者：
Ghisaidoobe AB;Chung SJ
当供体荧光团和受体之间的距离在10 nm以内时发生Förster共振能量转移（FRET），其应用通常需要对生物靶标进行荧光标记。然而，生物分子的共价修饰可能无意中引起构象和/或功能变化。本文综述了主要来源于色氨酸的内源性蛋白质荧光（λEX = 280 nm，λEM = 350 nm）在蛋白质相关研究中的应用，重点介绍了无标记FRET技术。在波长和强度方面，色氨酸荧光受到其（或蛋白质）局部环境的强烈影响，除了荧光猝灭外，还被应用于研究蛋白质构象变化。内源性Förster共振能量转移（iFRET）是一种新近发展起来的技术，它利用色氨酸的内源性荧光，结合靶特异性荧光探针分别作为FRET供体和受体，对天然蛋白质进行真实的实时检测。
Förster resonance energy transfer (FRET) occurs when the distance between a donor fluorophore and an acceptor is within 10 nm, and its application often necessitates fluorescent labeling of biological targets. However, covalent modification of biomolecules can inadvertently give rise to conformational and/or functional changes. This review describes the application of intrinsic protein fluorescence, predominantly derived from tryptophan (λEX ∼ 280 nm, λEM ∼ 350 nm), in protein-related research and mainly focuses on label-free FRET techniques. In terms of wavelength and intensity, tryptophan fluorescence is strongly influenced by its (or the protein’s) local environment, which, in addition to fluorescence quenching, has been applied to study protein conformational changes. Intrinsic Förster resonance energy transfer (iFRET), a recently developed technique, utilizes the intrinsic fluorescence of tryptophan in conjunction with target-specific fluorescent probes as FRET donors and acceptors, respectively, for real time detection of native proteins.
登录
查看更多内容
影响因子：
1.7
作者：
Ghosh, Saptarshi;Paul, Bijan Kumar;Chattopadhyay, Nitin
通讯作者：
Chattopadhyay, Nitin
影响因子：
3.3
作者：
Gasymov, Oktay K.;Abduragimov, Adil R.;Glasgow, Ben J.
通讯作者：
Glasgow, Ben J.
影响因子：
2.8
作者：
Callis, PR;Vivian, JT
通讯作者：
Vivian, JT
影响因子：
5.6
作者：
BAKER, EN
通讯作者：
BAKER, EN
影响因子：
1.1
作者：
GUDGIN, E;LOPEZDELGADO, R;WARE, WR
通讯作者：
WARE, WR