Arabidopsis NUCLEAR FACTOR Y A8 inhibits the juvenile-to-adult transition by activating transcription of MIR156s
Arabidopsis NUCLEAR FACTOR Y A8 inhibits the juvenile-to-adult transition by activating transcription of MIR156s
复制标题
拟南芥核因子 Y A8 通过激活 MIR156 转录抑制幼体向成体的转变
DOI：
10.1093/jxb/eraa197
复制
发表时间：
2020
影响因子：
6.9
通讯作者：
Li Gang
中科院分区：
文献类型：
--
作者：
Zhao Hang;Lin Ke;Ma Lin;Chen Qingshuai;Gan Shuo;Li Gang
营养（幼年到成年）和开花（营养到生殖）阶段的变化在高等植物的生活史中至关重要。microRNA 156（miR 156）及其靶基因鳞状细胞启动子结合蛋白样（SPL）基因是决定营养生长期变化的主要调节因子。随着植物衰老，miR 156水平逐渐下降，并且其表达迅速被糖抑制。然而，MIR 156基因转录调控的潜在调控机制仍不清楚。在本研究中，我们证明了拟南芥核因子Y A8（NF-YA 8）直接与多个MIR 156基因启动子中的CCAAT顺式元件结合，从而激活它们的转录并抑制幼叶向成叶的转变。我们的研究结果表明，NF-YA 8作为一个信号枢纽，整合内部发育年龄和糖信号，调节转录的MIR 156，从而影响幼年到成年和开花的过渡。
Vegetative (juvenile-to-adult) and flowering (vegetative-to-reproductive) phase changes are crucial in the life cycle of higher plants. MicroRNA156 (miR156) and its targetSQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL) genes are master regulators that determine vegetative phase changes. The miR156 level gradually declines as a plant ages and its expression is rapidly repressed by sugar. However, the underlying regulatory mechanism of transcriptional regulation of theMIR156gene remains largely unknown. In this study, we demonstrated that Arabidopsis NUCLEAR FACTOR Y A8 (NF-YA8) binds directly to CCAATcis-elements in the promoters of multipleMIR156genes, thus activating their transcription and inhibiting the juvenile-to-adult transition.NF-YA8was highly expressed in juvenile-stage leaves, and significantly repressed with developmental age and by sugar signals. Our results suggest that NF-YA8 acts as a signaling hub, integrating internal developmental age and sugar signals to regulate the transcription ofMIR156s, thus affecting the juvenile-to-adult and flowering transitions.