UPTAKE AND CONVERSION OF THE ANTIBIOTIC ALBOMYCIN BY ESCHERICHIA-COLI K-12
UPTAKE AND CONVERSION OF THE ANTIBIOTIC ALBOMYCIN BY ESCHERICHIA-COLI K-12
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DOI：
10.1111/j.1432-1033.1979.tb13283.x
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
BRAUN, V
中科院分区：
文献类型：
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作者：
HARTMANN, A;FIEDLER, HP;BRAUN, V
抗生素白霉素通过与供铁铁铬络合物相同的摄取系统被运送到大肠杆菌K-12的细胞中。白霉素和铁铬铁络合异羟甲酸铁的部分结构相似。在快速铁摄取阶段，细胞内没有发现螯合剂。为了了解白霉素的抗菌活性，用氚标记了白霉素，并在推测的抗菌活性区域用放射性S标记了白霉素。虽然氚标记不被细胞保留，但S标记的部分被拿起并在细胞内浓缩了500倍。由于S被回收为低分子量组分，因此它没有被结合到蛋白质或核酸中。Bio-Gel P-2凝胶过滤显示，孵育液中有1个氚标记的切割产物和2个S标记的切割产物。显然，白霉素通过其类铁铬部分主动转运到细胞内，而抑制生长的部分可能通过细胞内的水解释放并保留在细胞内。
The antibiotic albomycin is transported into cells of E. coli K-12 by the same uptake system as the Fe-supplying ferrichrome complex. The Fe-complexing hydroxamate moieties of albomycin and ferrichrome are structurally similar. During the phase of rapid Fe uptake the chelators were not found in the cells. To understand the antibiotic activity of albomycin, it was labeled in the hydroxamate with tritium and in the presumed antibiotically active area with radioactive S. While the tritium label was not retained by the cells, part of the S label was taken up and concentrated 500-fold within the cell. The S was not incorporated into proteins or nucleic acids since it was recovered as a low MW component. Gel filtration on Bio-Gel P-2 revealed 1 tritium-labeled and 2 S-labeled cleavage products in the incubation medium. Albomycin is apparently actively transported via its ferrichrome-like portion into the cells and the growth-inhibitory moiety may be released by hydrolysis intracellularly and remain there.