AUTOLYTIC PROCESSING OF DIMERIC PLANT-VIRUS SATELLITE RNA

AUTOLYTIC PROCESSING OF DIMERIC PLANT-VIRUS SATELLITE RNA
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DOI:
10.1126/science.231.4745.1577
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发表时间:
1986-03-28
期刊:
影响因子:
56.9
通讯作者:
BRUENING, G
BRUENING, G
中科院分区:
综合性期刊1区
文献类型:
--
作者:
PRODY, GA;BAKOS, JT;BRUENING, G

文献摘要

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与一些植物病毒相关的是小卫星RNA,它们依赖植物病毒提供保护性外壳蛋白,并且可能至少提供卫星RNA复制所需的一些蛋白质。烟草环斑病毒卫星RNA的多聚体形式可能是单体卫星RNA的体内前体。证据表明该卫星 RNA 的二聚体和三聚体可在体外进行自溶加工。该反应生成具有生物活性的单体卫星RNA,显然可以可逆地从单体RNA形成二聚体RNA,并且不需要酶进行催化。
Associated with some plant viruses are small satellite RNA''s that depend on the plant virus to provide protective coat protein and presumably at least some of the proteins necessary for satellite RNA replication. Multimeric forms of the satellite RNA of tobacco ringspot virus are probable in vivo precursors of the monomeric satellite RNA. Evidence is presented for the in vitro autolytic processing of dimeric and trimeric forms of this satellite RNA. The reaction generates biologically active monomeric satellite RNA, apparently is reversible to form dimeric RNA from monomeric RNA, and does not require an enzyme for its catalysis.