The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration
The effect of integrin-specific bioactive coatings on tissue healing and implant osseointegration
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DOI：
10.1016/j.biomaterials.2008.03.036
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发表时间：
2008-07-01
期刊：
影响因子：
14
通讯作者：
Garcia, Andres J.
中科院分区：
文献类型：
--
作者：
Petrie, Timothy A.;Raynor, Jenny E.;Garcia, Andres J.
种植体骨整合是骨科和牙科临床成功的必要条件，它被定义为骨对位和功能固定，但许多应用都受到种植体松动的限制。对植入物进行修饰以呈现生物活性基序，如来自纤维连接蛋白(FN)的RGD细胞黏附序列，代表了再生医学中一种有前途的方法。然而，这些仿生策略在体内组织愈合方面只产生了轻微的增强作用。在这项研究中，临床级的钛种植体被移植了一种无污染的寡聚(乙二醇基)取代的聚合物涂层，其具有不同的靶向整合素受体的特异性配体的密度。与未修饰的钛和RGD呈递表面相比，呈现α(5)β(1)整合素特异性FN片段FNIII7-10的生物材料促进了骨髓基质细胞的成骨分化。重要的是，与RGD功能化和未修饰的钛相比，FNIII7-10功能化的钛在体内显著改善了功能性种植体骨整合。这项研究表明，促进整合素结合特异性的生物活性涂层调节骨髓来源的祖细胞成骨细胞分化，并增强生物医学植入物的愈合反应和功能整合。这项工作为再生医学生物材料的合理设计确定了一种创新战略。(C)2008爱思唯尔有限公司。保留所有权利。
Implant osseointegration, defined as bone apposition and functional fixation, is a requisite for clinical success in orthopaedic and dental applications, many of which are restricted by implant loosening. Modification of implants to present bioactive motifs such as the RGD cell-adhesive sequence from fibronectin (FN) represents a promising approach in regenerative medicine. However, these biomimetic strategies have yielded only marginal enhancements in tissue healing in vivo. In this study, clinical-grade titanium implants were grafted with a non-fouling oligo(ethylene glycol)-substituted polymer coating functionalized with controlled densities of ligands of varying specificity for target integrin receptors. Biomaterials presenting the alpha(5)beta(1)-integrin-specific FN fragment FNIII7-10 enhanced osteoblastic differentiation in bone marrow stromal cells compared to unmodified titanium and RGD-presenting surfaces. Importantly, FNIII7-10-functionalized titanium significantly improved functional implant osseointegration compared to RGD-functionalized and unmodified titanium in vivo. This study demonstrates that bioactive coatings that promote integrin binding specificity regulate marrow-derived progenitor osteoblastic differentiation and enhance healing responses and functional integration of biomedical implants. This work identifies an innovative strategy for the rational design of biomaterials for regenerative medicine. (C) 2008 Elsevier Ltd. All rights reserved.