Biomechanical changes after repeated collagen cross-linking on human corneas assessed in vitro using scanning acoustic microscopy.
Biomechanical changes after repeated collagen cross-linking on human corneas assessed in vitro using scanning acoustic microscopy.
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使用扫描声学显微镜在体外评估人角膜上重复胶原交联后的生物力学变化。
DOI：
10.1167/iovs.13-13042
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发表时间：
2014-03-13
影响因子：
4.4
通讯作者：
Radhakrishnan H
中科院分区：
文献类型：
--
作者：
Beshtawi IM;Akhtar R;Hillarby MC;O'Donnell C;Zhao X;Brahma A;Carley F;Derby B;Radhakrishnan H
利用扫描声学显微镜（SAM）观察反复交联引起的生物力学变化。将30个人角膜分为三组。A组5只角膜交联1次。在B组中，5个角膜交联两次，间隔24小时。在C组中，5个角膜交联3次，间隔24小时。所有组中的对侧对照具有类似的处理，但没有UV-A。使用SAM评估声速，其与组织的弹性模量的平方根成正比。在A组中，治疗角膜的声速为前1677.38 ± 10.70 ms−1和后1603.90 ± 9.82 ms−1，而对照角膜的声速为前1595.23 ± 9.66 ms−1和后1577.13 ± 8.16 ms−1。在B组中，治疗角膜的声速为前1746.33 ± 23.37 ms−1和后1631.60 ± 18.92 ms±1，而对照角膜的声速为前1637.57 ± 22.15 ms±1和后1612.30 ± 22.23 ms−1。在C组中，治疗角膜的声速为前1717.97 ± 18.92 ms−1和后1616.62 ± 17.58 ms−1，而对照组的声速为前1628.69 ± 9.37 ms−1和后1597.68 ± 11.97 ms−1。A、B和C组中交联角膜和对照角膜之间前部（200 × 200 μm）区域的声速分别增加了1.051（P = 0.005）、1.066（P = 0.010）和1.055（P = 0.005）倍。然而，所有组中的交联角膜之间没有显著差异（P = 0.067）。与对照组相比，在所有处理组中发现声速显著增加;然而，处理组之间的差异不显著，表明当重复胶原交联处理时，不会诱导进一步的交联。
To explore the biomechanical changes induced by repeated cross-linking using scanning acoustic microscopy (SAM). Thirty human corneas were divided into three groups. In group A, five corneas were cross-linked once. In group B, five corneas were cross-linked twice, 24 hours apart. In group C, five corneas were cross-linked three times, 24 hours apart. The contralateral controls in all groups had similar treatment but without UV-A. The speed of sound, which is directly proportional to the square root of the tissue’s elastic modulus, was assessed using SAM. In group A, the speed of sound of the treated corneas was 1677.38 ± 10.70 ms−1 anteriorly and 1603.90 ± 9.82 ms−1 posteriorly, while it was 1595.23 ± 9.66 ms−1 anteriorly and 1577.13 ± 8.16 ms−1 posteriorly in the controls. In group B, the speed of sound of the treated corneas was 1746.33 ± 23.37 ms−1 anteriorly and 1631.60 ± 18.92 ms±1 posteriorly, while it was 1637.57 ± 22.15 ms±1 anteriorly and 1612.30 ± 22.23 ms−1 posteriorly in the controls. In group C, the speed of sound of the treated corneas was 1717.97 ± 18.92 ms−1 anteriorly and 1616.62 ± 17.58 ms−1 posteriorly, while it was 1628.69 ± 9.37 ms−1 anteriorly and 1597.68 ± 11.97 ms−1 posteriorly in the controls. The speed of sound in the anterior (200 × 200 μm) region between the cross-linked and control corneas in groups A, B, and C was increased by a factor of 1.051 (P = 0.005), 1.066 (P = 0.010), and 1.055 (P = 0.005) respectively. However, there was no significant difference among the cross-linked corneas in all groups (P = 0.067). A significant increase in speed of sound was found in all treated groups compared with the control group; however, the difference among the treated groups is not significant, suggesting no further cross-links are induced when collagen cross-linking treatment is repeated.
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影响因子：
4.2
作者：
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通讯作者：
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