Monitoring newly synthesized proteins over the adult life span of Caenorhabditis elegans.
Monitoring newly synthesized proteins over the adult life span of Caenorhabditis elegans.
复制标题
DOI：
10.1021/acs.jproteome.5b00021
复制
发表时间：
2015-03-06
影响因子：
4.4
通讯作者：
Miyagi, Masaru
中科院分区：
文献类型：
--
作者：
Vukoti, Krishna;Yu, Xiaokun;Sheng, Quanhu;Saha, Sudipto;Feng, Zhaoyang;Hsu, Ao-Lin;Miyagi, Masaru
关于个体蛋白质的合成和降解在生物体生命期间如何变化，我们知之甚少。这些知识对于理解衰老过程至关重要。为了填补这一知识空白，我们监测新合成的蛋白质在秀丽隐杆线虫的蛋白质组规模随着时间的推移，在成年期使用SILAC为基础的标签追逐的方法。对于大多数蛋白质，新合成蛋白质的出现率在成年的前5天很高，在第5天和第11天之间减慢，然后在第11天之后再次增加。然而，出现率的大小显着不同的蛋白质。例如，参与胚胎发育、转录调节和脂质结合/转运的蛋白质的新合成蛋白质的出现是快速的，在成年后的第5天，这些蛋白质中有>70%是新合成的，而参与细胞组装和运动的蛋白质（如肌动蛋白和肌球蛋白）的出现是缓慢的，即使在第16天，这些蛋白质中也有<70%是新合成的。新合成的蛋白质的寿命后期增加尤其是核糖体蛋白和ATP酶。我们还研究了RNAi介导的rpl-9（核糖体蛋白），atp-3（ATP合成酶）和ril-1（RNAi诱导的长寿-1）基因敲低的效果，发现从成年后期开始抑制atp-3和ril-1的表达仍然有效地延长了C.优美的
Little is known regarding how the synthesis and degradation of individual proteins changes during the life of an organism. Such knowledge is vital to understanding the aging process. To fill this knowledge gap, we monitored newly synthesized proteins on a proteome scale in Caenorhabditis elegans over time during adulthood using a SILAC-based label-chase approach. For most proteins, the rate of appearance of newly synthesized protein was high during the first 5 days of adulthood, slowed down between the fifth and the 11th days, and then increased again after the 11th day. However, the magnitude of appearance rate differed significantly from protein to protein. For example, the appearance of newly synthesized protein was fast for proteins involved in embryonic development, transcription regulation, and lipid binding/transport, with >70% of these proteins newly synthesized by day 5 of adulthood, whereas it was slow for proteins involved in cellular assembly and motility, such as actin and myosin, with <70% of these proteins newly synthesized even on day 16. The late-life increase of newly synthesized protein was especially high for ribosomal proteins and ATP synthases. We also investigated the effect of RNAi-mediated knockdown of the rpl-9 (ribosomal protein), atp-3 (ATP synthase), and ril-1 (RNAi-induced longevity-1 ) genes and found that inhibiting the expression of atp-3 and ril-1 beginning in late adulthood is still effective to extend the life span of C. elegans.
登录
查看更多内容
影响因子：
7.8
作者：
Ching TT;Paal AB;Mehta A;Zhong L;Hsu AL
通讯作者：
Hsu AL
影响因子：
7.8
作者：
Reis-Rodrigues P;Czerwieniec G;Peters TW;Evani US;Alavez S;Gaman EA;Vantipalli M;Mooney SD;Gibson BW;Lithgow GJ;Hughes RE
通讯作者：
Hughes RE
影响因子：
4.4
作者：
Sheng, Quanhu;Dai, Jie;Zeng, Rong
通讯作者：
Zeng, Rong
影响因子：
9.8
作者：
David DC;Ollikainen N;Trinidad JC;Cary MP;Burlingame AL;Kenyon C
通讯作者：
Kenyon C
影响因子：
4.5
作者：
Hansen M;Hsu AL;Dillin A;Kenyon C
通讯作者：
Kenyon C