Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1
Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1
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DOI：
10.1016/j.celrep.2015.08.063
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发表时间：
2015-10-06
期刊：
影响因子：
8.8
通讯作者：
Kiyono, Hiroshi
中科院分区：
文献类型：
--
作者：
Kunisawa, Jun;Sugiura, Yuki;Kiyono, Hiroshi
生物能量代谢在细胞分化过程中发生变化，但B细胞代谢的细节尚不清楚。在这里，我们展示了B细胞在肠道分化过程中的代谢变化，B细胞分化为IgA（+）浆细胞（PCs）。Peyer’s patches （PPs）中的Naive B细胞和iLP中的IgA(+) PCs细胞都进行三羧酸（TCA）循环，但只有IgA(+) PCs细胞进行糖酵解。这些代谢差异反映了它们对维生素B-1的依赖，维生素B-1是TCA循环的重要辅助因子。事实上，在饮食中维生素B1缺失后，TCA循环活性的降低减少了PPs中幼稚B细胞的数量，但不影响iLP中的IgA(+) PCs。需要通过膳食维生素B1来维持幼稚B细胞，以诱导（但不是维持）肠道对口服抗原的IgA反应。这些发现揭示了饮食介导的B细胞免疫代谢在有组织和弥漫性肠组织中的维持。
Bioenergetic metabolism varies during cell differentiation, but details of B cell metabolism remain unclear. Here, we show the metabolic changes during B cell differentiation in the intestine, where B cells differentiate into IgA(+) plasma cells (PCs). Naive B cells in the Peyer's patches (PPs) and IgA(+) PCs in the intestinal lamina propria (iLP) both used the tricarboxylic acid (TCA) cycle, but only IgA(+) PCs underwent glycolysis. These metabolic differences reflected their dependencies on vitamin B-1, an essential cofactor for the TCA cycle. Indeed, the diminished activity of the TCA cycle after dietary vitamin B1 depletion decreased the number of naive B cells in PPs without affecting IgA(+) PCs in the iLP. The maintenance of naive B cells by dietary vitamin B1 was required to induce-but not maintain-intestinal IgA responses against oral antigens. These findings reveal the diet-mediated maintenance of B cell immunometabolism in organized and diffuse intestinal tissues.