Cloning of a human "epidermal-type" 12-lipoxygenase-related gene and chromosomal localization to 17p13.
Cloning of a human "epidermal-type" 12-lipoxygenase-related gene and chromosomal localization to 17p13.
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人类“表皮型”12-脂氧合酶相关基因的克隆和染色体定位至 17p13。
DOI：
10.1159/000014993
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Funk,CD
中科院分区：
文献类型：
--
作者：
Sun,D;Elsea,SH;Patel,PI;Funk,CD
用小鼠e-12 LO cDNA探针克隆了人表皮型12-脂氧合酶（e-12 LO）假基因。与其它脂氧合酶基因相似，基因组序列显示14个外显子和13个内含子，覆盖6.7kb的DNA。通过RACE-PCR从人皮肤和毛囊的RNA中扩增出对应于该基因的cDNA，并显示出与三种鼠12-脂氧合酶cDNA的65-80%同一性。基于几个标准，人类似乎缺乏功能性的e-12 LO基因，而是获得了表达的假基因。通过体细胞杂种DNA分析，将假基因ALOX 12 P2定位于染色体17 p13。
A human “epidermal-type” 12-lipoxygenase (e-12LO) pseudogene was cloned using a murine e-12LO cDNA probe. Similar to other lipoxygenase genes, the genomic sequence revealed 14 exons and 13 introns covering 6.7 kb of DNA. cDNA corresponding to this gene was amplified from RNA of human skin and hair follicles by RACE-PCR and displayed 65–80% identity to the three murine 12-lipoxygenase cDNAs. Based on several criteria, humans appear to lack a functional e-12LO gene but instead have acquired an expressed pseudogene. The pseudogene designated ALOX12P2 was localized to chromosome 17p13 by analysis of somatic hybrid DNA panels.